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Recombinant DNA

Recombinant DNA

Viral Recombination

Viral Recombination

Cells are sometimes infected by more than one virus at once. When two viruses disassemble to expose their genomes for replication in the same cell, similar regions of their genomes can pair together and exchange sequences in a process called recombination. Alternatively, viruses with segmented genomes can swap segments in a process called reassortment.

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Related Experiment Video

Updated: Jun 22, 2026

Simplified Reverse Genetics Method to Recover Recombinant Rotaviruses Expressing Reporter Proteins

Simplified Reverse Genetics Method to Recover Recombinant Rotaviruses Expressing Reporter Proteins

Published on: April 17, 2020

A simple reverse genetics method to generate recombinant coronaviruses.

Julien Mélade¹, Géraldine Piorkowski¹, Franck Touret¹

¹Unité des Virus Émergents (UVE: Aix-Marseille Univ-IRD 190-Inserm 1207), Marseille, France.

|March 3, 2022

Summary

A new infectious subgenomic amplicons (ISA) method simplifies engineering severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and other coronaviruses. This technique facilitates rapid generation of viral variants and reporter strains for research and therapeutic development.

Keywords:

SARS-CoV-2 antivirals in vivo experiment reverse genetics serology

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Last Updated: Jun 22, 2026

Simplified Reverse Genetics Method to Recover Recombinant Rotaviruses Expressing Reporter Proteins

Simplified Reverse Genetics Method to Recover Recombinant Rotaviruses Expressing Reporter Proteins

Published on: April 17, 2020

Rapid, Seamless Generation of Recombinant Poxviruses using Host Range and Visual Selection

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Published on: May 24, 2020

Reverse Genetics to Engineer Positive-Sense RNA Virus Variants

Reverse Genetics to Engineer Positive-Sense RNA Virus Variants

Published on: June 9, 2022

Area of Science:

Virology
Molecular Biology
Genetics

Background:

Engineering recombinant viruses is crucial for studying emerging pathogens like SARS-CoV-2.
The large genome size of coronaviruses presents challenges for existing reverse genetics techniques.

Purpose of the Study:

To introduce a simplified method for generating infectious recombinant coronaviruses.
To apply this method to SARS-CoV-2 and feline enteric coronavirus.
To enable the creation of specific viral variants and reporter strains.

Main Methods:

Development and application of the "infectious subgenomic amplicons" (ISA) technology.
Generation of recombinant coronaviruses without complete genomic cDNA reconstruction.
Incorporation of mutations and reporter genes into the SARS-CoV-2 genome.

Main Results:

Successful rescue of wild-type SARS-CoV-2 and feline enteric coronavirus with original strain characteristics.
Demonstrated ability to generate specific SARS-CoV-2 genomic variants.
Created fluorescent reporter strains of SARS-CoV-2 for various applications.

Conclusions:

The ISA method offers a swift and simple approach to coronavirus reverse genetics.
This technique can accelerate studies on SARS-CoV-2 variants and their properties.
Facilitates the development of diagnostic tools and therapeutic reagents for coronaviruses.