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Published on: March 17, 2019
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Long non-coding RNAs in Sus scrofa ileum under starvation stress
Shu Wang1, Yi Jia Ma1, Yong Shi Li1
1Department of Animal Sciences, Shanxi Agricultural University, Taigu, Shanxi Province 030801, China.
Animal Bioscience
|March 3, 2022
Summary
This study identified novel long non-coding RNAs (lncRNAs) involved in piglet starvation stress. These findings provide a foundation for understanding starvation mechanisms and developing strategies to alleviate stress.
Area of Science:
- Genomics
- Molecular Biology
- Animal Science
Background:
- Starvation stress significantly impacts animal physiology.
- Long non-coding RNAs (lncRNAs) are increasingly recognized for their regulatory roles in cellular processes.
- Understanding lncRNA involvement in stress responses is crucial for animal health and welfare.
Purpose of the Study:
- To identify long non-coding RNAs (lncRNAs) associated with starvation stress in piglets.
- To analyze the functional roles of these lncRNAs and their target genes.
- To discover potential molecular targets for mitigating starvation stress.
Main Methods:
- Generation of a piglet starvation stress model with short-term (48h) and long-term (72h) starvation groups.
- High-throughput sequencing of lncRNA libraries from piglet ileums.
- Bioinformatic analysis including differential expression, target prediction, functional enrichment, and network construction.
Main Results:
- Identification of 11,792 lncRNAs, including 2,500 novel ones.
- Discovery of 509 differentially expressed lncRNAs (DElncRNAs) under starvation stress.
- lncRNA-targeted genes are primarily involved in metabolic, cellular, immune, digestive, and transport processes.
Conclusions:
- Specific lncRNAs, including MSTRG.19894.13, MSTRG.16726.3, and MSTRG.12176.1, are implicated in starvation stress.
- The study generated a valuable lncRNA library for piglets.
- Provides a foundation for screening key lncRNAs and further research into starvation stress mechanisms.
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