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Updated: Oct 1, 2025
An Orthotopic Mouse Model of Spontaneous Breast Cancer Metastasis
Published on: August 14, 2016
Development of New Mouse Breast Cancer Model of Local Bone Metastasis and Verification Using Bisphosphonates
Ryo Shoji1, Hiroyuki Tsuchie2, Hiroyuki Nagasawa2
1Department of Orthopedic Surgery, Akita University Graduate School of Medicine, Akita, Japan yebyayayeby@gmail.com.
Background/Aim:
Local tumor injection models require complicated procedures. The purpose was to establish a simple local bone metastasis model using normal mice, and to study the usefulness of the model with bisphosphonates (BP).
Materials And Methods:
This study used a versatile C57BL/6 mouse model and E0771 cells. Tumor cells were injected into the right femur. Mice were divided into groups depending on the concentration of cells injected and the use of BP or not. The degree of bone destruction between the different conditions was compared using micro-computed tomography (μCT).
Results:
Bone destruction was confirmed in four mice in the high-concentration group at 3 weeks, and in all other mice at 4 and 6 weeks. At 6 weeks post-injection, bone destruction was significantly suppressed in the BP group (p<0.05).
Conclusion:
We created a breast cancer mouse model of local bone metastasis. Zoledronate showed the same usefulness as in previous models. It may be an effective model for evaluating treatments for bone metastasis.
