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Down-regulation of AMPD3 Is Associated With Poor Survival in Head and Neck Squamous Cell Carcinoma
Cheng-Ming Hsu1,2, Shun-Fu Chang3, Yao-Te Tsai1
1Department of Otolaryngology-Head and Neck Surgery, Chang Gung Memorial Hospital, Chiayi, Taiwan, R.O.C.
Background:
Adenosine monophosphate deaminase 3 (AMPD3) is an isoenzyme involved in the regulation of the energetic metabolism of mammalian cells. Cancer cells have a high demand for their energy supply. This experimental study aimed to illustrate the role of AMPD3 in human head and neck squamous cell carcinoma (HNSCC).
Materials And Methods:
Real-time quantitative reverse transcription-polymerase chain reaction was used to investigate the expression of the AMPD3 gene in human HNSCC tissues to assess the changes in cancerous and noncancerous parts and the correlation with different tumor behavior. The functions of AMPD3 were investigated using wound-healing and migration assays.
Results:
AMPD3 was significantly down-regulated in cancerous tissues of HNSCC (p=0.001) and this was correlated with more advanced tumor and clinical stages. Patients with high expression had better 5-year survival. AMPD3 knock-down in SCC-4 and SCC-25 cells demonstrated reduction of proliferation but increased migration and invasion.
Conclusion:
To our knowledge, this is the first report evidencing the expression pattern of AMPD3 in HNSCC and demonstrated that high AMPD3 expression might represent a good prognostic biomarker. AMPD3 may have an antiproliferative potential but its down-regulation may not contribute to reducing the migration and invasion of HNSCC cells.
Insights
Adenosine monophosphate deaminase 3 (AMPD3) is down-regulated in head and neck squamous cell carcinoma (HNSCC). High AMPD3 expression correlates with better survival, suggesting it may be a prognostic biomarker.
Area of Science:
- Biochemistry
- Molecular Biology
- Oncology
Background:
- Adenosine monophosphate deaminase 3 (AMPD3) regulates cellular energy metabolism.
- Cancer cells exhibit high energy demands.
- The role of AMPD3 in head and neck squamous cell carcinoma (HNSCC) requires elucidation.
Purpose of the Study:
- To investigate the expression pattern of AMPD3 in HNSCC.
- To determine the correlation between AMPD3 expression and HNSCC tumor behavior.
- To explore the functional role of AMPD3 in HNSCC cell lines.
Main Methods:
- Real-time quantitative reverse transcription-polymerase chain reaction (RT-qPCR) for gene expression analysis.
- Analysis of AMPD3 expression in cancerous versus noncancerous HNSCC tissues.
- Wound-healing and migration assays to assess cell function.
Main Results:
- AMPD3 was significantly down-regulated in HNSCC tissues (p=0.001).
- Lower AMPD3 expression correlated with advanced tumor and clinical stages.
- AMPD3 knockdown reduced proliferation but increased migration and invasion in HNSCC cells.
Conclusions:
- This study is the first to report AMPD3 expression patterns in HNSCC.
- High AMPD3 expression may serve as a favorable prognostic biomarker for HNSCC.
- AMPD3 exhibits antiproliferative potential, but its down-regulation may not impede HNSCC cell migration and invasion.
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