Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cardiac Monitoring with Textile Capacitive Electrodes in Driving Applications: Characterization of Signal Quality and RR Duration Accuracy.

Sensors (Basel, Switzerland)·2025
Same author

Respiratory Monitoring with Textile Inductive Electrodes in Driving Applications: Effect of Electrode's Positioning and Form Factor on Signal Quality.

Sensors (Basel, Switzerland)·2025
Same author

A Quantitative Method to Guide the Integration of Textile Inductive Electrodes in Automotive Applications for Respiratory Monitoring.

Sensors (Basel, Switzerland)·2024
Same author

Development of a high-speed imaging system for real time evaluation and monitoring of cardiac engineered tissues.

Frontiers in bioengineering and biotechnology·2024
Same author

In silico study of multicellular automaticity of heterogeneous cardiac cell monolayers: Effects of automaticity strength and structural linear anisotropy.

PLoS computational biology·2018
Same author

The heart in lack of oxygen? A revisited method to improve cardiac performance ex vivo.

American journal of physiology. Heart and circulatory physiology·2018

Related Experiment Video

Updated: Oct 1, 2025

Discrimination and Characterization of Heterocellular Populations Using Quantitative Imaging Techniques
09:48

Discrimination and Characterization of Heterocellular Populations Using Quantitative Imaging Techniques

Published on: June 30, 2017

7.6K

Novel Analysis Method for Beating Cells Videomicroscopy Data: Functional Characterization of Culture Samples.

Jonathan Béland1,2, James Elber Duverger1,3, Philippe Comtois1,2,3

  • 1Research Centre, Montreal Heart Institute, Montreal, QC, Canada.

Frontiers in Physiology
|March 4, 2022
PubMed
Summary

This study introduces novel videomicroscopy techniques to analyze cardiac tissue contractility in vitro. These methods assess contraction dynamics and synchronicity, improving cardiotoxicity screening for drug development.

Keywords:
cardiomyocyte monolayercontractile activityheterogeneityimaging analysisnon-linear analysisspatial-temporal activity

More Related Videos

Workflow for High-content, Individual Cell Quantification of Fluorescent Markers from Universal Microscope Data, Supported by Open Source Software
09:57

Workflow for High-content, Individual Cell Quantification of Fluorescent Markers from Universal Microscope Data, Supported by Open Source Software

Published on: December 16, 2014

13.1K
Kinetic Visualization of Single-Cell Interspecies Bacterial Interactions
08:33

Kinetic Visualization of Single-Cell Interspecies Bacterial Interactions

Published on: August 5, 2020

7.1K

Related Experiment Videos

Last Updated: Oct 1, 2025

Discrimination and Characterization of Heterocellular Populations Using Quantitative Imaging Techniques
09:48

Discrimination and Characterization of Heterocellular Populations Using Quantitative Imaging Techniques

Published on: June 30, 2017

7.6K
Workflow for High-content, Individual Cell Quantification of Fluorescent Markers from Universal Microscope Data, Supported by Open Source Software
09:57

Workflow for High-content, Individual Cell Quantification of Fluorescent Markers from Universal Microscope Data, Supported by Open Source Software

Published on: December 16, 2014

13.1K
Kinetic Visualization of Single-Cell Interspecies Bacterial Interactions
08:33

Kinetic Visualization of Single-Cell Interspecies Bacterial Interactions

Published on: August 5, 2020

7.1K

Area of Science:

  • Biomedical Engineering
  • Cardiovascular Research
  • Regenerative Medicine

Background:

  • In vitro cardiac models are crucial for cardiotoxicity screening and regenerative medicine.
  • Current methods often focus on activity rate, limiting comprehensive assessment.
  • There is a need for advanced techniques to evaluate multicellular electrophysiological and contractile properties.

Purpose of the Study:

  • To develop new videomicroscopy-based approaches for analyzing cardiac cell culture contractility.
  • To introduce novel variables for quantifying contraction dynamics and rhythm dependency.
  • To present methods for assessing regional synchronicity and identifying contractile heterogeneity.

Main Methods:

  • Utilizing videomicroscopy to capture monolayer cardiac cell activity.
  • Developing new analytical variables for contraction dynamics.
  • Implementing image analysis for regional synchronicity evaluation.

Main Results:

  • The study proposes two new variables linked to contraction dynamics and rhythm.
  • Methods for evaluating regional synchronicity within cardiac cell cultures are presented.
  • These approaches can rapidly identify abnormal activity and heterogeneity.

Conclusions:

  • New videomicroscopy techniques offer enhanced analysis of cardiac cell contractility in vitro.
  • These methods improve the assessment of drug effects on cardiac tissue models.
  • The developed approaches support automated screening and better understanding of cardiac function.