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Updated: Oct 1, 2025

A Precision Medicine Tool for Measurement and Monitoring of Hemoglobin S in Sickle Cell Disease Patients Receiving Transfusion Therapy
Erythrocyte (red blood cell) dataset in thalassemia case
Dyah Aruming Tyas1, Tri Ratnaningsih2, Agus Harjoko1
1Department of Computer Science and Electronics, Universitas Gadjah Mada, Yogyakarta, Indonesia.
Abstract:
Red blood cell (RBC) dataset was obtained from four thalassemia peripheral blood smears and a healthy peripheral blood smear. The dataset contains 7108 images of individual red blood cells for nine cell types. The first process is image acquisition, which is the process of retrieving microscopic image data from peripheral blood smears through a Olympus CX21 microscope using an Optilab advance plus camera. Laboratory assistants helped obtain ideal erythrocyte images. We provide peripheral blood smear from four thalassemia patients in the ThalassemiaPBS dataset. After image acquisition, the image is resized from 4100 × 3075 pixels to 800 × 600 pixels to reduce the computing load in the next stage. We extracted the green color component (green channel) of the RGB image and used it in the next process. We chose the green channel because it is not affected by variations in color and brightness. Furthermore, the segmentation stage is carried out to obtain an object in the form of a single red blood cell. After that, the object can be classified according to the type of red blood cell. This dataset can become an opportunity for international researchers to develop the classification method for red blood cells.
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