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Updated: Aug 13, 2026
10:19
A Murine Model of Group B Streptococcus Vaginal Colonization
Published on: November 16, 2016
Monoclonal antibodies produced to Streptococcus agalactiae
American Journal of Veterinary Research
|June 1, 1986
Summary
Two monoclonal antibodies targeting Streptococcus agalactiae demonstrated high specificity and sensitivity. This research proposes a new method for detecting Streptococcus agalactiae infection in milk samples.
Area of Science:
- Veterinary Microbiology
- Immunology
- Bacteriology
Background:
- Streptococcus agalactiae (Group B Streptococcus) is a significant pathogen in cattle, causing mastitis and economic losses.
- Accurate and rapid diagnostic methods are crucial for controlling Streptococcus agalactiae outbreaks in dairy herds.
- Existing diagnostic techniques may have limitations in specificity or sensitivity.
Purpose of the Study:
- To develop and evaluate monoclonal antibodies for the specific detection of Streptococcus agalactiae.
- To assess the sensitivity and specificity of these antibodies against common bovine pathogens.
- To propose a diagnostic procedure utilizing monoclonal antibodies for identifying Streptococcus agalactiae in milk.
Main Methods:
- Production of monoclonal antibodies against Streptococcus agalactiae.
- Enzyme-linked immunosorbent assay (ELISA) to test antibody reactivity.
- Indirect fluorescent antibody test (IFAT) for antibody detection.
- Testing against Streptococcus agalactiae and other relevant gram-positive bacteria from dairy herds.
Main Results:
- Both monoclonal antibodies showed strong reactivity with Streptococcus agalactiae in ELISA and IFAT.
- The antibodies did not cross-react with other frequently encountered gram-positive organisms in Mississippi dairy herds.
- This indicates high specificity for Streptococcus agalactiae.
Conclusions:
- Monoclonal antibodies offer a specific and sensitive tool for Streptococcus agalactiae detection.
- A proposed procedure using these antibodies can aid in the diagnosis of Streptococcus agalactiae-infected milk.
- This approach has the potential to improve mastitis control strategies in dairy cattle.
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