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Related Experiment Video

Updated: Oct 1, 2025

Preparation of Whole Bone Marrow for Mass Cytometry Analysis of Neutrophil-lineage Cells
08:09

Preparation of Whole Bone Marrow for Mass Cytometry Analysis of Neutrophil-lineage Cells

Published on: June 19, 2019

9.5K

Mass cytometry staining for human bone marrow clinical samples.

Margaret Hallisey1, Jenna Dennis1, Charlotte Abrecht1

  • 1Department of Medical Oncology, Center for Immuno-Oncology, Dana-Farber Cancer Institute, 450 Brookline Avenue, Boston, MA 02215, USA.

STAR Protocols
|March 4, 2022
PubMed
Summary
This summary is machine-generated.

This protocol optimizes mass cytometry staining for human bone marrow immune cells. It overcomes challenges like low cell viability to enable detailed immunophenotyping of immune populations in clinical samples.

Keywords:
Cell BiologyCell-based AssaysFlow Cytometry/Mass CytometryHealth SciencesImmunology

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Area of Science:

  • Immunology
  • Biotechnology
  • Hematology

Background:

  • Human bone marrow analysis is crucial for understanding immune cell function.
  • Challenges in bone marrow processing include reduced cell viability and low cell counts.
  • Accurate immunophenotyping requires robust protocols for handling fragile samples.

Purpose of the Study:

  • To detail an optimized staining protocol for mass cytometry immunophenotyping of human bone marrow immune cells.
  • To address and overcome common limitations associated with human bone marrow sample processing.
  • To enable comprehensive characterization of immune cell states in clinical bone marrow samples.

Main Methods:

  • Development of a specialized staining technique for mass cytometry.
  • Optimization of sample preparation to maintain cell viability and integrity.
  • Validation of the protocol using human bone marrow samples.

Main Results:

  • Successful acquisition of single, viable immune cells from human bone marrow.
  • The protocol effectively mitigates issues of reduced viability and fragile cell pellets.
  • Demonstrated ability to analyze immune cell activation, exhaustion, and cytotoxicity.

Conclusions:

  • This optimized protocol provides a reliable method for immunophenotyping human bone marrow immune populations.
  • The technique ensures high-quality data acquisition for downstream analyses.
  • Facilitates comprehensive immune profiling of clinical bone marrow samples for research and diagnostics.