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Flow-cytometry-based protocol to analyze respiratory chain function in mouse microglia.

Daniel Erny^1,2, Nikolaos Dokalis^1,3, Charlotte Mezö^1,3

¹Institute of Neuropathology, University of Freiburg, Freiburg, Germany.

|March 4, 2022

Summary

This summary is machine-generated.

This study introduces a flow cytometry protocol to measure respiratory chain function in mouse microglia ex vivo. This method allows for efficient analysis of cellular metabolism in isolated cells.

Keywords:

Cell Biology Cell isolation Flow Cytometry/Mass Cytometry Immunology Metabolism Neuroscience

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Assessing Retinal Microglial Phagocytic Function In Vivo Using a Flow Cytometry-based Assay

Assessing Retinal Microglial Phagocytic Function In Vivo Using a Flow Cytometry-based Assay

Published on: October 18, 2016

Area of Science:

Immunology
Cell Biology
Biochemistry

Background:

Current metabolic analysis protocols often rely on in vitro cell cultures.
Ex vivo analysis of cellular metabolism, particularly in microglia, presents technical challenges.

Purpose of the Study:

To develop and present a flow cytometry-based protocol for assessing respiratory chain function in ex vivo mouse microglia.
To provide a method applicable to other isolated cell types and cell cultures.

Main Methods:

Microglial cell isolation from mouse tissue.
Flow cytometry analysis of respiratory chain complexes I and II in permeabilized cells.
Optimization for low cell input.

Main Results:

Successful measurement of respiratory chain function in permeabilized mouse microglia ex vivo.
Demonstration of a protocol requiring minimal cell input.

Conclusions:

The presented flow cytometry protocol offers an efficient method for analyzing ex vivo microglial respiratory function.
This protocol is adaptable for various cell types, enhancing cellular metabolism research.