Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Immune Surveillance by NK Cells and Phagocytes01:25

Immune Surveillance by NK Cells and Phagocytes

4.2K
Immune surveillance is an integral part of the innate immune system, involving the continuous monitoring of peripheral tissues to detect and respond to pathogens, infected cells, or cancerous cells. This surveillance is conducted primarily by natural killer (NK) cells and phagocytes, which employ distinct but complementary mechanisms to identify and eliminate threats.
Natural Killer Cells: The Fast Responders
NK cells are large granular lymphocytes found in the blood and lymphatic system. These...
4.2K
Chemotaxis and Direction of Cell Migration01:21

Chemotaxis and Direction of Cell Migration

3.7K
Cells can detect chemical cues in their environment and reorganize the cytoskeleton to migrate toward them or away from them. This directional migration, called chemotaxis, is essential during embryogenesis and development, immune response, tissue repair and regeneration, and reproduction. These chemical cues can either attract or repel the cell's movement. For example, axon development is determined by a combination of chemoattractants and chemorepellents that direct the growing axon...
3.7K
Inflammation01:38

Inflammation

55.9K
Overview
55.9K
Cells of the Innate Immune Response01:28

Cells of the Innate Immune Response

4.7K
The innate immune response is an immediate and non-specific response against pathogens, acting swiftly to prevent the spread of infections. The primary cells involved in this response are phagocytes and natural killer (NK) cells.
Phagocytes
Phagocytes police the peripheral tissues by removing cellular debris and responding to the invasion of foreign substances or pathogens. Many phagocytes attack and remove microorganisms even before lymphocytes detect them. The human body has two general...
4.7K
Structure and Function of Leukocytes01:21

Structure and Function of Leukocytes

2.5K
An adult in good health typically has between 4,500 and 11,000 leukocytes, or white blood cells, per microliter of blood, which constitutes about 1% of the total blood volume. Unlike red blood cells, white blood cells contain a nucleus and other cellular organelles but do not have hemoglobin. Most white blood cells reside in connective tissues, particularly in lymphatic organs such as the lymph nodes, with only a small fraction present in circulating blood.
White blood cells protect the body...
2.5K
Classification of Leukocytes01:30

Classification of Leukocytes

3.4K
Leukocytes are classified into two groups based on the presence or absence of cytoplasmic granules. Granular leukocytes, which contain granules, belong to the myeloid lineage and are divided into three subtypes: neutrophils, eosinophils, and basophils. These cells are roughly spherical and characterized by the granules in their cytoplasm.
Neutrophils are the most abundant type of granular leukocytes, comprising 50-70% of all leukocytes. They feature small, evenly distributed granules and a...
3.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Monocyte reprogramming by nociceptors impedes T cell-mediated tumor immunity.

bioRxiv : the preprint server for biology·2026
Same author

Modeling lymph node metastases in vivo.

Nature protocols·2025
Same author

Intestinal CD4<sup>-</sup>CD8αβ<sup>-</sup>TCRαβ<sup>+</sup> T cells function as tolerogenic antigen presenting cells in mice.

Nature communications·2025
Same author

Setting the tone: nociceptors as conductors of immune responses.

Trends in immunology·2024
Same author

Primary nasal influenza infection rewires tissue-scale memory response dynamics.

Immunity·2024
Same author

Lung injury-induced activated endothelial cell states persist in aging-associated progressive fibrosis.

Nature communications·2024

Related Experiment Video

Updated: Oct 1, 2025

Imaging the Neutrophil Phagosome and Cytoplasm Using a Ratiometric pH Indicator
12:42

Imaging the Neutrophil Phagosome and Cytoplasm Using a Ratiometric pH Indicator

Published on: April 5, 2017

10.2K

Quo vadis, neutrophil?

Rodrigo J Gonzalez1, Ulrich H von Andrian1

  • 1Department of Immunology, Harvard Medical School, Boston, MA, USA; The Ragon Institute of MGH, MIT and Harvard, Cambridge, MA, USA.

Cell
|March 4, 2022
PubMed
Summary

A serotonin metabolite, 5-HIAA, unexpectedly recruits neutrophils to inflamed tissues. Produced by platelets and mast cells, it activates the GPR35 receptor, highlighting a new pathway in inflammation and host defense.

Area of Science:

  • Immunology
  • Cell Biology
  • Pharmacology

Background:

  • Neutrophil recruitment is crucial for inflammation and fighting microbes.
  • The mechanisms governing neutrophil migration to inflamed sites are complex and not fully understood.

Purpose of the Study:

  • To investigate the role of serotonin metabolites in neutrophil recruitment.
  • To identify novel mediators involved in inflammatory responses.

Main Methods:

  • Investigated the function of 5-hydroxyindoleacetic acid (5-HIAA) in neutrophil migration.
  • Utilized cellular assays to examine the interaction between 5-HIAA and the GPR35 receptor.
  • Studied the production of 5-HIAA by activated platelets and mast cells.

Main Results:

More Related Videos

Isolation and Characterization of Neutrophils with Anti-Tumor Properties
10:15

Isolation and Characterization of Neutrophils with Anti-Tumor Properties

Published on: June 19, 2015

25.0K
ATAC-Seq Library Preparation of Murine Bone Marrow-Derived Neutrophils
09:44

ATAC-Seq Library Preparation of Murine Bone Marrow-Derived Neutrophils

Published on: January 3, 2025

653

Related Experiment Videos

Last Updated: Oct 1, 2025

Imaging the Neutrophil Phagosome and Cytoplasm Using a Ratiometric pH Indicator
12:42

Imaging the Neutrophil Phagosome and Cytoplasm Using a Ratiometric pH Indicator

Published on: April 5, 2017

10.2K
Isolation and Characterization of Neutrophils with Anti-Tumor Properties
10:15

Isolation and Characterization of Neutrophils with Anti-Tumor Properties

Published on: June 19, 2015

25.0K
ATAC-Seq Library Preparation of Murine Bone Marrow-Derived Neutrophils
09:44

ATAC-Seq Library Preparation of Murine Bone Marrow-Derived Neutrophils

Published on: January 3, 2025

653
  • Identified 5-HIAA, a serotonin metabolite, as a key mediator of neutrophil recruitment.
  • Demonstrated that 5-HIAA is produced by activated platelets and mast cells.
  • Showed that 5-HIAA engages the orphan receptor GPR35 to induce neutrophil migration to inflamed tissues.

Conclusions:

  • 5-HIAA plays an unanticipated but significant role in neutrophil recruitment during inflammation.
  • The 5-HIAA/GPR35 axis represents a novel pathway in inflammatory processes and host defense.
  • Targeting this pathway could offer new therapeutic strategies for inflammatory diseases.