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The epitranscriptome toolbox.

Sharon Moshitch-Moshkovitz1, Dan Dominissini1, Gideon Rechavi1

  • 1Cancer Research Center, Sheba Medical Center, Tel Hashomer, Israel; Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel; Wohl Institute for Translational Medicine, Sheba Medical Center, Tel Hashomer, Israel.

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|March 4, 2022
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Summary
This summary is machine-generated.

Messenger RNA (mRNA) modifications regulate gene expression. This primer reviews tools for detecting and studying various RNA modifications, including the prevalent N6-methyladenosine (m⁶A).

Keywords:
RNA modificationsepitranscriptomicsmass spectrometrytranscriptome

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Area of Science:

  • Molecular Biology
  • Epigenetics
  • Gene Expression Regulation

Background:

  • Messenger RNA (mRNA) modifications play a crucial role in regulating gene expression.
  • Thousands of studies have established the significance of these modifications over the past decade.
  • Advancements in technology enable precise identification and mapping of RNA modifications across the transcriptome.

Purpose of the Study:

  • To provide a technical overview of available tools and approaches for studying RNA modifications.
  • To highlight methods for detecting and characterizing diverse RNA modifications.
  • To discuss the evolution of techniques, particularly for N6-methyladenosine (m⁶A) and other modifications.

Main Methods:

  • Review of existing and emerging technologies for RNA modification detection.
  • Discussion of transcriptome-wide mapping techniques.
  • Functional characterization approaches for RNA marks.

Main Results:

  • A growing number of RNA modifications can now be accurately identified and mapped.
  • Specific methods have been developed for prevalent modifications like m⁶A.
  • Unique properties of other RNA modifications have driven the development of specialized approaches.

Conclusions:

  • Current technologies offer significant insights into the biology of RNA modifications.
  • A diverse toolkit is available for researchers studying various RNA modifications.
  • The field continues to evolve, with new methods emerging for comprehensive analysis.