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Updated: Oct 1, 2025
Development of a Direct Pulp-capping Model for the Evaluation of Pulpal Wound Healing and Reparative Dentin Formation in Mice
Published on: January 12, 2017
Pulpal and periapical tissue response after direct pulp capping with endosequence root repair material and low-level
Loai Alsofi1, Wafaa Khalil2, Nada O Binmadi3
1Department of Endodontics, Faculty of Dentistry, King Abdulaziz University, P.O.Box 80209, Jeddah, 21589, Saudi Arabia. lalsofi@kau.edu.sa.
Background:
The study aims to investigate the pulp and periapical reaction and healing after capping with EndoSequence Root Repair Material (ERRM) combined with low-level laser application.
Methods:
In 6 rabbits, pulps were exposed via class V, half of the samples received a low-level diode laser at 980 nm. Thereafter, cavities were capped with regular-set ERRM. The specimens were processed for histomorphological examination after 2 weeks and two months.
Results:
After 2 weeks, images show mild inflammation and organized odontoblasts in lased group. The non-lased group shows more severe inflammation. The predentin thickness was thicker in the lased group with statistical significance (p < 0.05). After 2 months, inflammatory cells were sparse in both lased and non-lased groups. In the periapical area, group one showed dilated blood vessels and thick fibrous connective tissues. In group two, there were more numerous maturations of PDL fibers with scattered inflammatory cells and congested blood vessel.
Conclusions:
Using low-level laser therapy in combination with ERRM for pulp capping shortens the inflammatory phase and enhances healing.
