Impregnating biochar with Fe and Cu by bioleaching for fabricating catalyst to activate H₂O₂

Biochar is an excellent support material for heterogeneous catalyst in Fenton reaction. However, fabrication of heterogeneous catalyst supported by biochar normally adopts chemical impregnation which is costly and difficult in post-treatment. Here, impregnation by bioleaching driven by Acidithiobacillus ferrooxidans was developed. Bioleaching was particularly effective in loading iron to biochar. Iron loading amount was 225.5 mg/g after 10-g biochar was treated in bioleaching containing 40-g FeSO₄·7H₂O for 60 h. When copper was added into bioleaching, simultaneous impregnation with iron and copper could be achieved. Impregnation mechanism for iron was jarosite formation on biochar surface and adsorption for copper. For the high metal content, after pyrolysis, the final composites could activate hydrogen peroxide to decolorize dye effectively. With 15 mg as-synthesized Cu-Fe@biochar containing 254.3 mg/g iron and 33.4 mg/g copper, 50 mg/L reactive red 3BS or methylene blue could be decolorized completely in 20 min in a 100-mL solution by 16-mM H₂O₂ at pH 2.5. Compared with existing impregnation methods, bioleaching was facile, cheap and green, and deserved more concern. KEY POINTS: • High amount of Fe is loaded to biochar uniformly as jarosite by bioleaching. • Cu is adsorbed onto biochar during bioleaching. • Synthesized Cu-Fe@biochar is an excellent photo-Fenton catalyst.