Rapid screening of microalgae by a 96-hole air-flowing device

In this study, a 96-hole air-flowing device (96HAFD) was established for high-throughput screening of three mutant Chlorella strains under air aeration. 96HAFD was first tested for the confirmation of homogeneous air aeration cultivation environment at 1.2 L min^-1 for algal screening based on the results of t test (p < 0.05) in the verification of consistency experiment. Then the data of dynamic growth characteristics of three mutant Chlorella strains indicated the good agreement in three screening devices including 96HAFD, flask and tube air-flowing cultivation devices by linear regression analysis between the 96HAFD and tube (R² = 0.9904), 96HAFD and flask (R² = 0.9904). At last, the 96HAFD was verified more efficient and reliable in fast screening single algal colony strains when compared with flask and tube air-flowing cultivation devices, because 96HAFD was confirmed have better performances in adaptation to the aeration cultivation circumstance and growing faster in a short period, in addition, 96HAFD had the less percentage of water loss per day (0.11%) than that of flask aeration device (2-3%) and tube aeration device (5-6.5%), which reduced negative effect caused by the water evaporation in the aeration cultivation to make the whole growing system more stable.