Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A label-free electrochemical aptasensor enables ultrasensitive and specific detection of neurofilament light.

Biosensors & bioelectronics·2026
Same author

Expedient single-round selection of hyper-modified aptamer targeting insulin receptor from over-represented dually nucleobase-modified DNA libraries.

Nature communications·2026
Same author

Charge Injection and Interfiber Electrical Conduction in Cable Bacteria.

ACS applied materials & interfaces·2026
Same author

Site-Selective Electrodeposition on a Microelectrode Array Chip for Parallel Detection of <i>Staphylococcus aureus</i> and <i>Escherichia coli</i>.

Analytical chemistry·2026
Same author

Pullulan Coating Preserves High Conductivity in Cable Bacteria Wires.

ACS applied bio materials·2026
Same author

Just a little prick: careful cell contacts enabled by ceramic nanostraws.

Pflugers Archiv : European journal of physiology·2026

Related Experiment Video

Updated: Oct 1, 2025

In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses
12:23

In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses

Published on: September 7, 2022

1.8K

DNA aptamer selection for SARS-CoV-2 spike glycoprotein detection.

Mateo Alejandro Martínez-Roque1, Pablo Alberto Franco-Urquijo1, Víctor Miguel García-Velásquez1

  • 1Laboratorio de Terapia Génica, Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del I.P.N., CDMX, 07360, Mexico.

Analytical Biochemistry
|March 5, 2022
PubMed
Summary

Researchers developed DNA aptamers to detect the SARS-CoV-2 Spike glycoprotein. These aptamers enabled sensitive electrochemical and fluorescent aptasensors for rapid virus detection, crucial for public health.

Keywords:
AptamersAptasensorCOVID-19Capillary electrophoresisSARS-CoV-2SELEX

More Related Videos

Engineering Antiviral Agents via Surface Plasmon Resonance
13:00

Engineering Antiviral Agents via Surface Plasmon Resonance

Published on: June 14, 2022

2.5K
High-throughput Confocal Imaging of Quantum Dot-Conjugated SARS-CoV-2 Spike Trimers to Track Binding and Endocytosis in HEK293T Cells
06:39

High-throughput Confocal Imaging of Quantum Dot-Conjugated SARS-CoV-2 Spike Trimers to Track Binding and Endocytosis in HEK293T Cells

Published on: April 21, 2022

3.2K

Related Experiment Videos

Last Updated: Oct 1, 2025

In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses
12:23

In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses

Published on: September 7, 2022

1.8K
Engineering Antiviral Agents via Surface Plasmon Resonance
13:00

Engineering Antiviral Agents via Surface Plasmon Resonance

Published on: June 14, 2022

2.5K
High-throughput Confocal Imaging of Quantum Dot-Conjugated SARS-CoV-2 Spike Trimers to Track Binding and Endocytosis in HEK293T Cells
06:39

High-throughput Confocal Imaging of Quantum Dot-Conjugated SARS-CoV-2 Spike Trimers to Track Binding and Endocytosis in HEK293T Cells

Published on: April 21, 2022

3.2K

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Analytical Chemistry

Background:

  • The global spread of SARS-CoV-2 necessitated rapid development of diagnostic tools.
  • Existing detection methods require improvement in cost-effectiveness and throughput.
  • Specific recognition elements are vital for accurate SARS-CoV-2 detection.

Purpose of the Study:

  • To isolate and validate DNA aptamers targeting the SARS-CoV-2 Spike glycoprotein.
  • To develop sensitive and specific aptasensors for detecting the SARS-CoV-2 Spike glycoprotein.
  • To provide cost-effective diagnostic solutions for SARS-CoV-2.

Main Methods:

  • Ideal-Filter Capillary Electrophoresis SELEX (IFCE-SELEX) for aptamer selection.
  • Next Generation Sequencing (NGS) for aptamer characterization.
  • Binding assays and aptasensor development (electrochemical and fluorescent) for validation.

Main Results:

  • Isolation of two specific DNA aptamers, C7 and C9, targeting the SARS-CoV-2 Spike glycoprotein.
  • Development of electrochemical and fluorescent aptasensors with high sensitivity.
  • Achieved detection limits of 0.07 fM for electrochemical and 41.87 nM for fluorescent aptasensors.

Conclusions:

  • Selected DNA aptamers (C7, C9) are effective recognition elements for SARS-CoV-2 detection.
  • Developed aptasensors offer sensitive and specific detection of the SARS-CoV-2 Spike glycoprotein.
  • This approach provides a foundation for low-cost, high-throughput SARS-CoV-2 diagnostic assays.