Co-degradation of ofloxacin and its impact on solid phase denitrification with polycaprolactone as carbon source

Solid-phase denitrification has been applied for advanced nitrogen removal from wastewater and can co-degrade emerging pollutants. Fluoroquinolones (FQs), broad-spectral antibiotic, are frequently detected in the effluent of conventional wastewater treatment plants. However, it remains unclear whether solid-phase denitrifying bacteria can remove FQs. Thus, this study investigated the removal capacity of ofloxacin (OFX) as a representative of FQs and the microbial community structures of denitrifying sludge acclimated to polycaprolactone and OFX. The Results indicate that OFX had a negative effect on denitrification performance. OFX was degraded, and a possible pathway was revealed based on ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry. The dominant genera in the acclimated denitrifying sludge were Microbacterium, Simplicispira, Alicycliphilus, Reyranella, Sediminibacterium, Acidovorax and Thermomonas. Moreover, ABC transporters and cytochrome P450, related to multi-drug resistance and drug metabolism, were highly expressed in the acclimated sludge. This study provides novel insights into antibiotics control.