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A new network containing MYB109-ZmCesA5 is involved in kernel development.
Erfei Dong1, Chaoxian Liu1, Yang Bai1
1Maize Research Institute, Southwest University, Tiansheng Road, No. 2, Beibei District, Chongqing, China.
Biochemical and Biophysical Research Communications
|March 5, 2022
Summary
A newly identified MYB109-ZmCesA5 network regulates maize kernel development. This pathway impacts kernel weight by influencing starch and sucrose metabolism, highlighting a novel regulatory mechanism in maize.
Area of Science:
- Plant Biology
- Molecular Genetics
- Agricultural Science
Background:
- MYB transcription factors are crucial regulators of plant metabolism and development.
- The specific roles of MYB-CesA interactions in maize kernel development remain largely unexplored.
Purpose of the Study:
- To investigate the involvement of the MYB109-ZmCesA5 regulatory network in maize kernel development.
- To elucidate the molecular mechanisms by which this network influences kernel growth and metabolism.
Main Methods:
- Yeast one-hybrid (Y1H) and dual-luciferase reporter assays were used to confirm direct binding and transcriptional activation.
- Real-time quantitative PCR (RT-qPCR) and transcriptome analyses assessed gene expression changes in response to ZmMYB109 and ZmCesA5 manipulation.
- Phenotypic analysis of ZmCesA5 overexpression (OE) and knockout (KO) kernels was performed.
Main Results:
- ZmMYB109 directly binds to the ZmCesA5 promoter and activates its expression.
- ZmMYB109 expression is positively correlated with ZmCesA5 expression in developing kernels.
- Overexpression of ZmCesA5 leads to increased kernel weight, while ZmCesA5 loss-of-function impairs starch and sucrose metabolism, reducing kernel weight.
Conclusions:
- The MYB109-ZmCesA5 interaction represents a novel regulatory pathway in maize kernel development.
- This network plays a significant role in controlling kernel weight through modulation of carbohydrate metabolism.
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