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Related Concept Videos

Centrifugation01:05

Centrifugation

2.6K
Centrifugation is a separation technique based on differences in density or size. It is commonly used to separate solids from aqueous interferents. During centrifugation, the sample is placed in centrifugation tubes and spun at high angular velocity, which allows centrifugal force to act differentially on the different densities or masses of the components. After spinning, the supernatant liquid is decanted. Depending on the specific application, either the pellet or the supernatant is retained...
2.6K
Subcellular Fractionation01:32

Subcellular Fractionation

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The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
Differential Centrifugation
Differential centrifugation is...
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Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

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Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
6.4K

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Related Experiment Video

Updated: Oct 1, 2025

Preparation of Primary Acute Lymphoblastic Leukemia Cells in Different Cell Cycle Phases by Centrifugal Elutriation
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Improving cell viability using counterflow centrifugal elutriation.

Anqi Li1, Mehri Barabadi1, Hannah McDonald1

  • 1The Ritchie Centre, Hudson Institute of Medical Research, Melbourne, VIC, Australia; Department of Obstetrics and Gynaecology, Monash University, Melbourne, VIC, Australia.

Cytotherapy
|March 6, 2022
PubMed
Summary
This summary is machine-generated.

Counterflow centrifugal elutriation effectively removes dead cells, significantly improving cell viability in cell therapy manufacturing. This technology enhances product quality and manufacturing efficiency by optimizing cell separation processes.

Keywords:
automationbioprocessingcell manufacturingcell therapiescell viability

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Area of Science:

  • Biotechnology
  • Cell Therapy Manufacturing
  • Bioprocessing

Background:

  • Cell viability is a critical quality attribute for cell therapy products.
  • Low cell viability negatively impacts product quality and manufacturing efficiency.
  • Counterflow centrifugation is a cell separation technology used in cell therapy manufacturing.

Purpose of the Study:

  • To evaluate the efficacy of counterflow centrifugation for dead cell removal.
  • To improve the viability of cell therapy products.
  • To optimize counterflow centrifugation parameters for dead cell removal.

Main Methods:

  • Jurkat cells with varying dead cell loads were processed using counterflow centrifugal elutriation.
  • Process parameters (flow rate, centrifugal force) were correlated with outcomes (cell recovery, viability).
  • Optimized parameters were applied to T cells and human amniotic epithelial cells (hAECs).

Main Results:

  • Low flow rates in elutriation improved viable cell recovery.
  • Counterflow centrifugal processing increased T cell and hAEC viability (e.g., T cells from 80.67% to 94.73%).
  • T cell proliferation increased, while hAEC metabolic activity remained unchanged post-processing.

Conclusions:

  • Counterflow centrifugal elutriation is effective for dead cell removal.
  • This technology can be integrated into automated cell manufacturing protocols.
  • Dead cell removal via elutriation improves final product cell viability.