In situ generation of a Zwitterionic fluorescent probe for detection of human serum albumin protein

In this article, a new approach for human serum albumin selective fluorophore design has been reported. The fluorophore reported here comprises a substituted phenol donor and a cationic benzo[e]indolium acceptor connected with a π bond. Originally, the cationic fluorophore did not bind with human serum albumin. Upon deprotonation of the phenolic-OH by a water molecule the cationic form was transformed into an active zwitterionic form. Spectroscopic studies and theoretical calculations revealed that the new active form remained in a zwitterionic state in neutral aqueous solution, and it formed a strong supramolecular complex with human serum albumin. The spontaneous complexation resulted multi-fold increase of fluorescence intensity which increased linearly with the concentrations of the protein, thus giving an analytical tool to monitor human serum albumin in aqueous samples. We believe, this simple strategy applied on appropriate fluorogenic scaffolds would prove useful to develop new and improved turn-on fluorescent probes for pH regulated biological applications.