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Localization and function of tissue macrophages.

S Gordon, P R Crocker, L Morris

    Ciba Foundation Symposium
    |January 1, 1986
    PubMed
    Summary
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    The F4/80 antibody identifies macrophage populations in mice, revealing their distribution in tissues and distinct surface properties. This aids in understanding macrophage migration and interactions within various organs.

    Area of Science:

    • Immunology
    • Cell Biology
    • Developmental Biology

    Background:

    • The F4/80 antibody recognizes a specific glycoprotein on mature mouse macrophages.
    • Macrophage distribution and function are critical in tissue development, homeostasis, and disease.
    • Understanding macrophage heterogeneity is essential for immunological research.

    Purpose of the Study:

    • To characterize the F4/80 antigen and its expression on mouse macrophages.
    • To investigate macrophage distribution and migration patterns in various physiological and pathological conditions.
    • To explore the distinct surface properties of macrophages from different tissue compartments.

    Main Methods:

    • Utilized rat monoclonal antibody F4/80 for immunochemical analysis.
    • Employed cytochemistry and quantitative immunochemical techniques.

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  • Isolated macrophages from various mouse tissues (bone marrow, liver, spleen, peritoneal cavity, etc.).

    • Main Results:

    • Identified a ~160 kDa plasma membrane glycoprotein recognized by F4/80 on mature macrophages.
    • Mapped macrophage distribution in normal adult, embryonic, and infection models.
    • Demonstrated distinct surface properties between tissue-resident macrophages and circulating monocytes.

    Conclusions:

    • The F4/80 antigen is a valuable marker for studying mouse macrophage populations.
    • Macrophages exhibit ordered migration into developing and adult tissues.
    • Tissue-specific macrophage surface properties suggest roles in local cell adhesion and interactions.