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Related Concept Videos

Biofilms01:29

Biofilms

392
Biofilms are complex communities of microorganisms encased in a self-produced extracellular polysaccharide matrix attached to surfaces. These microbial consortia can include single or multiple species, providing enhanced survival benefits by forming organized, multilayered structures.The formation of biofilms occurs through four key stages: attachment, colonization, development, and dispersal.During attachment, free-swimming planktonic cells adhere to a surface, often facilitated by...
392

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Updated: Oct 1, 2025

Bile Salt-induced Biofilm Formation in Enteric Pathogens: Techniques for Identification and Quantification
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Consistent Biofilm Formation by Streptococcus pyogenes emm 1 Isolated From Patients With Necrotizing Soft Tissue

Dag Harald Skutlaberg1,2, Harald G Wiker1,2, Haima Mylvaganam2

  • 1Department of Clinical Science, University of Bergen, Bergen, Norway.

Frontiers in Microbiology
|March 7, 2022
PubMed
Summary

Streptococcus pyogenes emm1 isolates uniformly form biofilms, unlike other strains. The clinical impact of biofilm formation in necrotizing soft tissue infections remains unclear.

Keywords:
M-proteinStreptococcus pyogenesbiofilmsemm1necrotizing soft tissue infection (NSTI)

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Last Updated: Oct 1, 2025

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Published on: May 6, 2018

12.9K
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Area of Science:

  • Microbiology
  • Infectious Diseases
  • Molecular Biology

Background:

  • Biofilm formation is observed in Streptococcus pyogenes (necrotizing soft tissue infection) infections.
  • The role of M-protein in S. pyogenes biofilm formation is established, but emm type associations are inconsistent.
  • Clinical significance of S. pyogenes biofilms in necrotizing soft tissue infection (NSTI) requires further investigation.

Purpose of the Study:

  • Characterize biofilm-forming capacity of S. pyogenes isolates from NSTI patients.
  • Correlate biofilm formation with emm types and patient clinical characteristics.
  • Investigate the specific biofilm-forming behavior of emm1 isolates.

Main Methods:

  • Prospective observational study of 57 NSTI patients.
  • Collection of bacterial isolates and clinical data.
  • Microtiter plate assay to quantify biofilm-forming capacity.

Main Results:

  • emm1 isolates (22/57) showed uniform biofilm formation, distinct from other emm types (n=35).
  • emm1 isolates exhibited a narrow-ranged, normal distribution of biofilm capacity.
  • No significant association found between biofilm capacity and patient clinical features.

Conclusions:

  • emm1 isolates of S. pyogenes possess a distinct and uniform biofilm-forming capacity.
  • The clinical relevance of biofilm formation in S. pyogenes-associated NSTI remains to be determined.
  • Further research is needed to elucidate the precise role of biofilms in NSTI pathogenesis.