Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Editorial: Enhancing plant resilience and productivity through biostimulants and advanced biotechnological approaches.

Frontiers in plant science·2026

Same author

CircRNA expression profiling in H1N1-infected primary human tracheobronchial epithelial cells identifies candidate immune-related circRNAs validated in A549 cells.

Archives of virology·2026

Same author

Phenolic Compounds and Flavonoids in Various Extracts of <i>Ferula tadshikorum</i> and <i>Ferula sumbul</i> Grown in vitro Conditions.

Iranian journal of pharmaceutical research : IJPR·2026

Same author

Genomic and gene expression analyses of bacterium Pseudomonas sp. strain EB3 unravels plant growth-promoting activity under salinity stress.

Journal of applied microbiology·2025

Same author

Resilience of Portulaca plants to environmental stresses and the economic potential of their bioactive compounds.

Planta·2025

Same author

Genome-Wide Analysis of the FNSII Gene Family and the Role of CitFNSII-1 in Flavonoid Synthesis in Citrus.

Plants (Basel, Switzerland)·2025

Search research articles

Related Experiment Video

Updated: Oct 1, 2025

Annotation of Plant Gene Function via Combined Genomics, Metabolomics and Informatics

Annotation of Plant Gene Function via Combined Genomics, Metabolomics and Informatics

Published on: June 17, 2012

Small open reading frames in plant research: from prediction to functional characterization.

Sheue Ni Ong¹, Boon Chin Tan¹, Aisyafaznim Al-Idrus²

¹Centre for Research in Biotechnology for Agriculture (CEBAR), Universiti Malaya, 50603 Kuala Lumpur, Malaysia.

|March 7, 2022

Summary

Identifying small open reading frames (sORFs) in plants is crucial for crop improvement. This review addresses the lack of a comprehensive sORF annotation pipeline and discusses their classification, function, and applications.

Keywords:

Hormone-like peptides SEP Small open reading frame Small peptide sORFs

More Related Videos

mRNA Interactome Capture from Plant Protoplasts

mRNA Interactome Capture from Plant Protoplasts

Published on: July 28, 2017

Translating Ribosome Affinity Purification TRAP to Investigate Arabidopsis thaliana Root Development at a Cell Type-Specific Scale

Translating Ribosome Affinity Purification TRAP to Investigate Arabidopsis thaliana Root Development at a Cell Type-Specific Scale

Published on: May 14, 2020

Related Experiment Videos

Last Updated: Oct 1, 2025

Annotation of Plant Gene Function via Combined Genomics, Metabolomics and Informatics

Annotation of Plant Gene Function via Combined Genomics, Metabolomics and Informatics

Published on: June 17, 2012

mRNA Interactome Capture from Plant Protoplasts

mRNA Interactome Capture from Plant Protoplasts

Published on: July 28, 2017

Translating Ribosome Affinity Purification TRAP to Investigate Arabidopsis thaliana Root Development at a Cell Type-Specific Scale

Translating Ribosome Affinity Purification TRAP to Investigate Arabidopsis thaliana Root Development at a Cell Type-Specific Scale

Published on: May 14, 2020

Area of Science:

Genomics
Bioinformatics
Plant Science

Background:

Gene prediction is a challenging task.
Advancements in sequencing, ribosome profiling, and mass spectrometry enable small open reading frame (sORF) identification.
sORFs (< 100 codons) have been identified across various organisms for decades.

Purpose of the Study:

To address the absence of a comprehensive sORF annotation pipeline.
To review the classification and functions of plant sORFs.
To highlight the potential applications of sORFs in crop improvement.

Main Methods:

Review of existing literature on sORF identification and annotation.
Analysis of advancements in sequencing and proteomic technologies.
Compilation of information on plant sORF classification and function.

Main Results:

Sequencing and bioinformatics tools facilitate sORF discovery in plant genomes.
A comprehensive sORF annotation pipeline is currently lacking.
Plant sORFs exhibit diverse classifications and functions.

Conclusions:

Developing a comprehensive sORF annotation pipeline is a critical need.
Understanding plant sORF biology is essential for advancing crop genetics.
sORFs hold significant potential for enhancing crop improvement strategies.