Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Stage-specific insulin binding in mouse preimplantation embryos.

I Y Rosenblum, B A Mattson, S Heyner

    Developmental Biology
    |July 1, 1986
    PubMed
    Summary

    Insulin binding occurs specifically during later stages of mouse embryo development, starting at the morula and blastocyst stages. This binding is absent in earlier preimplantation stages, suggesting a crucial role for insulin during this critical period.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Quantitative gene expression analysis in a nonhuman primate model of antibiotic-induced nephrotoxicity.

    Toxicology and applied pharmacology·2004
    Same author

    Assessment of the sensitivity of the computational programs DEREK, TOPKAT, and MCASE in the prediction of the genotoxicity of pharmaceutical molecules.

    Environmental and molecular mutagenesis·2004
    Same author

    Quantitative PCR deconstruction of discrepancies between results reported by different hybridization platforms.

    Environmental health perspectives·2004
    Same author

    Comparison of two culture systems for the in-vitro growth and maturation of mouse preantral follicles.

    Clinical and experimental obstetrics & gynecology·2004
    Same author

    Serum troponins as biomarkers of drug-induced cardiac toxicity.

    Toxicologic pathology·2004
    Same author

    Assessment of temporal biochemical and gene transcription changes in rat liver cytochrome P450: utility of real-time quantitative RT-PCR.

    Pharmaceutical research·2003

    Area of Science:

    • Developmental Biology
    • Reproductive Biology
    • Endocrinology

    Background:

    • Insulin plays a vital role in mammalian physiology.
    • Its role during early embryonic development is not well understood.
    • Understanding insulin's function in preimplantation embryos is crucial for reproductive health.

    Purpose of the Study:

    • To investigate the presence and timing of insulin binding during mouse preimplantation development.
    • To determine if insulin binding is stage-specific in early mammalian embryos.

    Main Methods:

    • Indirect immunofluorescence technique was employed.
    • Antibodies against insulin were used for labeling.
    • Analysis was performed on unfertilized oocytes and embryos at 2-, 4-, 8-cell, morula, and blastocyst stages.

    Related Experiment Videos

    Main Results:

    • Insulin binding was observed in a stage-specific manner.
    • Concentration-dependent fluorescence labeling was detected in morula and blastocyst stages.
    • No insulin reactivity was found in unfertilized oocytes or 2-, 4-, and 8-cell embryos.

    Conclusions:

    • Insulin binding is specific to later stages of preimplantation mouse development.
    • This finding suggests a potential role for insulin in morula and blastocyst development.
    • This is the first report of stage-specific insulin binding during mammalian preimplantation development.