ATP2B1 gene rs71454161, rs73196661 and rs73196675 polymorphisms in eclampsia

Area of Science:

• Genetics
• Obstetrics
• Molecular Biology

Background:

• Eclampsia is a severe pregnancy complication.
• The role of specific gene polymorphisms in eclampsia is not fully understood.

Purpose of the Study:

• To investigate the association between ATP2B1 gene polymorphisms and eclampsia.
• To explore the correlation of these polymorphisms with gene expression and clinical indicators.

Main Methods:

• Case-control study with 150 eclampsia patients and 150 healthy controls.
• DNA extraction and sequencing of ATP2B1 gene polymorphisms (rs71454161, rs73196661, rs73196675).
• Analysis combined with Reverse Transcription-quantitative Polymerase Chain Reaction (RT-qPCR) for gene expression and clinical data.

Main Results:

• Significant differences in allele and genotype distributions of ATP2B1 polymorphisms (rs71454161, rs73196661, rs73196675) between groups.
• Specific alleles (G for rs71454161, T for rs73196661) and genotypes (GG for rs71454161, TT for rs73196661, CG for rs73196675) were more frequent in eclampsia patients.
• Polymorphisms correlated with ATP2B1 gene expression, 24-h urine protein, and lactate dehydrogenase (LDH) levels.

Conclusions:

• ATP2B1 gene polymorphisms are significantly associated with the occurrence and progression of eclampsia.
• These findings suggest ATP2B1 as a potential genetic factor in eclampsia pathogenesis.