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Updated: Oct 1, 2025
Standardized Colon Ascendens Stent Peritonitis in Rats - a Simple, Feasible Animal Model to Induce Septic Acute Kidney Injury
Published on: February 15, 2022
Irrigation of peritoneal cavity with cold atmospheric plasma treated solution effectively reduces microbial load in
Mustafa Onur Oztan1, Utku Kürşat Ercan2, Ayşegül Aksoy Gokmen3
1Department of Pediatric Surgery, School of Medicine, Izmir Katip Çelebi University, Izmir, 35620, Turkey. droztan@gmail.com.
Insights
Cold plasma-treated saline effectively combats intraperitoneal contamination and prevents sepsis in acute peritonitis models. This novel irrigation method shows promise for surgical management, offering a safer alternative to conventional techniques.
Area of Science:
- Medical research
- Surgical innovation
- Plasma medicine
Background:
- Appendicitis diagnosis in children is challenging, often leading to delays or misdiagnosis and perforation.
- Current surgical management for peritonitis involves appendectomy and peritoneal irrigation, but conventional methods face debate.
- Novel irrigation solutions are needed to improve outcomes and prevent sepsis.
Purpose of the Study:
- To investigate the efficacy of cold plasma-treated saline as an intraperitoneal irrigation solution for acute peritonitis.
- To assess the chemical and microbiological properties of plasma-treated saline.
- To evaluate the in-vivo safety and effectiveness of this novel irrigation method in a rat model.
Main Methods:
- Chemical and in vitro microbiological assessments of plasma-treated saline.
- Induction of acute peritonitis using the cecal ligation and perforation (CLP) model in 60 rats.
- In-vivo evaluation involving six groups, including sham operation, CLP, and various irrigation methods (saline, plasma-treated saline).
- Analysis of total antioxidant/oxidant status, oxidative stress index, microbiological, and pathological parameters.
Main Results:
- Plasma-treated saline was found to contain reactive species.
- Irrigation with plasma-treated saline effectively inactivated intraperitoneal contamination in the CLP model.
- The treatment successfully prevented sepsis development.
- No short-term local or systemic toxicity was observed with plasma-treated saline irrigation.
Conclusions:
- Cold plasma-treated saline is a potent antimicrobial agent suitable for intraperitoneal irrigation.
- This innovative approach offers a promising strategy for managing acute peritonitis and preventing sepsis.
- The method demonstrates safety and efficacy, addressing the need for improved irrigation techniques in surgery.
Abstract:
Accurate and timely diagnosis of appendicitis in children can be challenging, which leads to delayed admittance or misdiagnosis that may cause perforation. Surgical management involves the elimination of the focus (appendectomy) and the reduction of the contamination with peritoneal irrigation to prevent sepsis. However, the validity of conventional irrigation methods is being debated, and novel methods are needed. In the present study, the use of cold plasma treated saline solution as an intraperitoneal irrigation solution for the management of acute peritonitis was investigated. Chemical and in vitro microbiological assessments of the plasma-treated solution were performed to determine the appropriate plasma treatment time to be used in in-vivo experiments. To induce acute peritonitis in rats, the cecal ligation and perforation (CLP) model was used. Sixty rats were divided into six groups, namely, sham operation, plasma irrigation, CLP, dry cleaning after CLP, saline irrigation after CLP, and plasma-treated saline irrigation after CLP group. The total antioxidant and oxidant status, oxidative stress index, microbiological, and pathological evaluations were performed. Findings indicated that plasma-treated saline contains reactive species, and irrigation with plasma-treated saline can effectively inactivate intraperitoneal contamination and prevent sepsis with no short-term local and/or systemic toxicity.
