A DNA sequence containing the control sites for the uxaB gene of Escherichia coli

The nucleotide sequence of a 286 bp fragment containing the uxaB control region of Escherichia coli has been determined. The transcriptional start of the uxaB gene has been located and the promoter signals identified. Various fragments of the uxaB promoter-proximal region were fused in vitro with the lacZ gene. Results obtained with these fusions indicate that the operator-promoter sites are located on a 110 bp restriction fragment. The determination of the amino acid sequence of the NH2-terminus of the uxaB gene product revealed that the uxaB gene is not initiated with the AUG codon but with the unusual GTG codon. CRP, the cyclic AMP receptor protein, does not bind to the uxaB control region DNA even though expression of the uxaB gene is sensitive to catabolite repression.