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Fast detection of SARS-CoV-2 variants including Omicron using one-step RT-PCR and Sanger sequencing

Mandy Bloemen1, Annabel Rector1, Jill Swinnen1

  • 1KU Leuven, Rega Institute, Department of Microbiology, Immunology and Transplantation, Laboratory of Clinical and Epidemiological Virology, 3000 Leuven, Belgium.

Journal of Virological Methods
|March 8, 2022
PubMed

Keywords:
Easy detection of variants of concernRT-PCRSARS-CoV-2 OmicronSanger sequencing

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
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