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Complete Genome Sequence of Stenotrophomonas maltophilia Siphophage Suzuki

Teresa Sullivan1, Nikhil Manuel1, James Clark1,2

  • 1Department of Biochemistry and Biophysics, Texas A&M University, College Station, Texas, USA.

Microbiology Resource Announcements
|March 8, 2022
PubMed

Insights

We isolated and annotated the Suzuki siphophage, a virus that infects Stenotrophomonas maltophilia. Its genome shows similarities to other bacterial viruses, offering insights into phage diversity.

Area of Science:

  • Microbiology
  • Virology
  • Genomics

Background:

  • Stenotrophomonas maltophilia is a Gram-negative bacterium.
  • This bacterium is associated with human respiratory tract infections.

Purpose of the Study:

  • To isolate and perform genome annotation of the S. maltophilia siphophage Suzuki.
  • To analyze the genomic characteristics of the Suzuki phage.

Main Methods:

  • Bacteriophage isolation from environmental samples.
  • Whole-genome sequencing of the isolated phage.
  • Bioinformatic analysis for gene prediction and annotation.

Main Results:

  • Successful isolation and characterization of S. maltophilia siphophage Suzuki.
  • The phage genome is 56,042 base pairs long.
  • Annotation revealed 83 predicted protein-coding genes.
  • Genomic analysis showed similarity to Xylella phages Sano and Salvo.

Conclusions:

  • The Suzuki siphophage is a novel phage infecting Stenotrophomonas maltophilia.
  • Its genome shares features with other known bacteriophages.
  • This study contributes to understanding the diversity of S. maltophilia phages and their genetic makeup.

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