Sagittula salina sp. nov., isolated from marine waste

A novel Gram-stain-negative, non-motile, halophilic bacterium designated strain M10.9X^T was isolated from the inner sediment of an aluminium can collected from the Mediterranean Sea (València, Spain). Cells of strain M10.9X^T were rod-shaped and occasionally formed aggregates. The strain was oxidase-negative and catalase-positive, and showed a slightly psychrophilic, neutrophilic and slightly halophilic metabolism. The phylogenetic analyses revealed that strain M10.9X^T was closely related to Sagittula stellata E-37^T and Sagittula marina F028-2^T. The genomic G+C content of strain M10.9X^T was 65.2 mol%. The average nucleotide identity and digital DNA-DNA hybridization values were 76.6 and 20.9 %, respectively, confirming its adscription to a new species within the genus Sagittula. The major cellular fatty acids were C_18 : 1  ω7c/C_18 : 1  ω6c and C_16 : 0. The polar lipids consisted of phosphatidylglycerol, phosphatidylethanolamine, an unidentified aminolipid, an unidentified glycolipid, an unidentified phospholipid and an unidentified lipid. According to the resuts of a polyphasic study, strain M10.9X^T represents a novel species of the genus Sagittula for which the name Sagittula salina sp. nov. (type strain M10.9X^T=DSM 112301^T=CECT 30307^T) is proposed.