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Identification of MrgprD expression in mouse enteric neurons
Min Xu1, Zhudi Zhang1, Lei Lan2
1Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing, 210023, Jiangsu, People's Republic of China.
Cell and Tissue Research
|March 8, 2022
Summary
Mas-related G protein-coupled receptor D (MrgprD) is expressed in mouse enteric neurons, influencing gastrointestinal motility. This finding reveals MrgprD
Area of Science:
- Neuroscience
- Gastroenterology
- Molecular Biology
Background:
- Mas-related G protein-coupled receptor D (MrgprD) is known for its role in somatosensation, particularly pain and itch.
- Recent studies suggest MrgprD involvement in modulating murine intestinal motility.
- Previous detection of Mrgprd in the GI tract lacked cell-type specificity.
Purpose of the Study:
- To determine the specific cell types expressing MrgprD within the murine gastrointestinal tract.
- To elucidate the precise localization of MrgprD in the ileum and colon.
Main Methods:
- Utilized Mrgprd-tdTomato reporter mice.
- Employed whole-mount immunohistochemistry on ileum and colon tissues.
- Performed colocalization studies with NeuN staining to identify neuronal markers.
Main Results:
- tdTomato-positive cells, indicating MrgprD expression, were found in the myenteric plexus of the ileum and colon.
- These tdTomato-positive cells colocalized with NeuN-labeled enteric neurons.
- Direct immunohistochemistry confirmed MrgprD expression in these identified enteric neurons.
Conclusions:
- Mas-related G protein-coupled receptor D (MrgprD) is expressed in enteric neurons within the murine gastrointestinal tract.
- This expression highlights the potential role of MrgprD in GI physiology and pathophysiology.
- Further research into MrgprD's function in the gut is warranted.
