Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Transgenic Plants

Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...

Export of Mitochondrial and Chloroplast Genes

Export of Mitochondrial and Chloroplast Genes

A eukaryotic cell can have up to three different types of genetic systems: nuclear, mitochondrial, and chloroplast. During evolution, organelles have exported many genes to the nucleus; this transfer is still ongoing in some plant species. Approximately 18% of the Arabidopsis thaliana nuclear genome is thought to be derived from the chloroplast’s cyanobacterial ancestor, and around 75% of the yeast genome derived from the mitochondria’s bacterial ancestor. This export has occurred...

Plant Tissue Culture

Plant Tissue Culture

Plant tissue culture is widely used in both primary and applied science. Applications range from plant development studies to functional gene studies, crop improvement, commercial micropropagation, virus elimination, and conservation of rare species.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

WIP transcriptional regulators modulate developmental progression in both life cycle phases of a moss.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same author

Moss BRCA2 lacking the canonical DNA-binding domain promotes homologous recombination and binds to DNA.

Nucleic acids research·2025

Same author

Efficient and heritable gene editing through CRISPR-Cas9 in Pisum sativum.

Plant biotechnology journal·2025

Same author

Genome editing, an opportunity to revive soybean cultivation in Europe.

The Plant journal : for cell and molecular biology·2025

Same author

Establishing CRISPR-Cas9 in the sexually dimorphic moss, Ceratodon purpureus.

The Plant journal : for cell and molecular biology·2024

Same author

The SpRY Cas9 variant release the PAM sequence constraint for genome editing in the model plant Physcomitrium patens.

Transgenic research·2024

Search research articles

Related Experiment Video

Updated: Oct 1, 2025

Efficient Polyethylene Glycol PEG Mediated Transformation of the Moss Physcomitrella patens

Efficient Polyethylene Glycol PEG Mediated Transformation of the Moss Physcomitrella patens

Published on: April 19, 2011

Physcomitrium patens Protoplasting and Protoplast Transfection.

Florence Charlot¹, Guillaume Goudounet¹, Fabien Nogué¹

¹Institut Jean-Pierre Bourgin, INRAE, AgroParisTech, Université Paris-Saclay, Versailles, France.

Methods in Molecular Biology (Clifton, N.J.)

|March 8, 2022

Summary

This study standardizes protoplast production in Physcomitirella patens, enhancing genetic studies and bioengineering. A new alginate embedding method improves protoplast survival and regeneration efficiency.

Keywords:

Alginate CRISPR-Cas9 Homologous recombination PEG-mediated transfection Physcomitrella patens Protoplast Reverse genetics

More Related Videos

Scalable Transfection of Maize Mesophyll Protoplasts

Scalable Transfection of Maize Mesophyll Protoplasts

Published on: June 23, 2023

A Robotic Platform for High-throughput Protoplast Isolation and Transformation

A Robotic Platform for High-throughput Protoplast Isolation and Transformation

Published on: September 27, 2016

Related Experiment Videos

Last Updated: Oct 1, 2025

Efficient Polyethylene Glycol PEG Mediated Transformation of the Moss Physcomitrella patens

Efficient Polyethylene Glycol PEG Mediated Transformation of the Moss Physcomitrella patens

Published on: April 19, 2011

Scalable Transfection of Maize Mesophyll Protoplasts

Scalable Transfection of Maize Mesophyll Protoplasts

Published on: June 23, 2023

A Robotic Platform for High-throughput Protoplast Isolation and Transformation

A Robotic Platform for High-throughput Protoplast Isolation and Transformation

Published on: September 27, 2016

Area of Science:

Plant biology
Moss biotechnology
Molecular genetics

Background:

Physcomitrella patens protoplasts are crucial for reverse genetics and protein production.
Existing protocols require optimization for consistency and efficiency.

Purpose of the Study:

To present a standardized protocol for Physcomitirella patens protoplast production.
To detail transfection, regeneration, and selection methods.
To introduce an improved protoplast embedding technique.

Main Methods:

Standardized protoplast isolation from P. patens protonemata.
Procedures for protoplast transfection, plating, and selection.
Development of an alginate matrix for protoplast embedding and regeneration.

Main Results:

A consistent and standardized protocol for protoplast production and manipulation.
Successful transfection and regeneration of P. patens protoplasts.
Alleviation of warm agarized medium, optimizing transformed protoplast survival.

Conclusions:

The standardized protocol and alginate embedding enhance protoplast regeneration efficiency.
This method supports advanced genetic studies and bioengineering applications in P. patens.
The protocol offers a robust tool for moss biotechnology research.