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Updated: Oct 1, 2025

Fluorescence Activated Cell Sorting of Plant Protoplasts
Published on: February 18, 2010
Protoplast Preparation and Fluorescence-Activated Cell Sorting for the Evaluation of Targeted Mutagenesis in Plant
Ward Decaestecker1,2, Norbert Bollier1,2, Rafael Andrade Buono1,2
1Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium.
Abstract:
Fluorescence-activated cell sorting (FACS) allows for the enrichment of specific plant cell populations after protoplasting. In this book chapter, we describe the transformation and protoplasting of an Arabidopsis thaliana cell suspension culture (PSB-D, derived from MM2d) that can be used for the evaluation of CRISPR vectors in a subpopulation of cells. We also describe the protoplasting of Arabidopsis thaliana cells from the roots and stomatal lineage for the evaluation of tissue-specific gene editing. These protocols allow us to rapidly and accurately quantify various CRISPR systems in plant cells.
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