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Updated: Oct 1, 2025

Scalable Transfection of Maize Mesophyll Protoplasts
Published on: June 23, 2023
Characterization of Programmable Transcription Activators in the Model Monocot Setaria viridis Via Protoplast
Adam Sychla1,2,3, Juan Armando Casas-Mollano1,2,3, Matthew H Zinselmeier1,2,3
1Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Saint Paul, MN, USA.
Abstract:
Recent advances in DNA synthesis and assembly allow for genetic constructs to be designed and constructed in high throughput. Characterizing large numbers of variant genetic designs is not feasible with low-throughput and time-consuming plant transformation workflows. Protoplast transformation offers a rapid, high-throughput compatible alternative for testing genetic constructs in plant-relevant molecular environments. Here, we describe a protocol for protoplast transformation using a recent experiment in genetic optimization of dCas9-based programmable transcription activators as an example.
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