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Updated: Oct 1, 2025

Enrichment of Pachytene Spermatocytes and Spermatids from Mouse Testes Using Standard Laboratory Equipment
Published on: September 17, 2019
An experimental approach to comprehend the influence of platelet rich growth factors on spermatogenesis
Grigory A Demyashkin1,2, Tatyana G Borovaya3, Yulia Yu Andreeva4
1I.M. Sechenov First Moscow State Medical University (Sechenov University), Moscow, Russia.
Materials And Methods:
Experimental animals (n = 135) were divided into 5 groups: I - control (n = 10); II - 2IR (n = 35; 2 Gy); III - 2IR + LP-PRP + IGF-1 (n = 30); IV - 2IR + LP-PRP (n = 30); V - LP-PRP (n = 30).
Results:
Electron irradiation reduces the number of germ cells in comparison with the control group. After injection of LP-PRP + rhIGF-1 significantly increased the number of germ cells, Sertoli and Leydig cells, the height of germinal epithelium, area and diameter of seminiferous tubules.
Conclusion:
LP-PRP + rhIGF-1 has a normalizing effect on structural and functional disorders of the testis caused by electron irradiation.
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