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Biocatalytic membranes through aqueous phase separation.

Jéré J van Lente1, M Irshad Baig1, Wiebe M de Vos1

  • 1MESA+ Institute, Faculty of Science & Technology, University of Twente, Drienerlolaan 5, 7522 NB Enschede, the Netherlands.

Journal of Colloid and Interface Science
|March 8, 2022
PubMed
Summary

Aqueous phase separation (APS) enables sustainable production of biocatalytic membranes. This method easily incorporates enzymes like lysozyme, creating functional membranes with antibacterial properties and stable activity for over a week.

Keywords:
Aqueous phase separationBiocatalytic membrane(s)Enzyme(s)LysozymeMembrane(s)Polyelectrolyte complex(es)Polyelectrolyte(s)

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Area of Science:

  • Materials Science
  • Biotechnology
  • Chemical Engineering

Background:

  • Polymer membranes are essential in water treatment, chemical processing, and medicine.
  • Conventional membrane production uses unsustainable organic solvents.
  • Aqueous phase separation (APS) offers a sustainable alternative using polyelectrolyte complexation.

Purpose of the Study:

  • To demonstrate the first biocatalytic membrane produced via APS.
  • To showcase the facile incorporation of enzymes into APS-generated membranes.
  • To explore the potential of APS for creating functional polymer membranes.

Main Methods:

  • Utilized APS to create polyelectrolyte complex membranes.
  • Incorporated lysozyme into the membrane matrix during the APS process.
  • Characterized membrane structure, water permeability, and retention properties.
  • Assessed the antibacterial activity and enzymatic stability of the functionalized membranes.

Main Results:

  • Successfully produced the first biocatalytic membrane using APS.
  • Demonstrated easy incorporation of lysozyme without compromising membrane integrity or performance.
  • Functionalized membranes exhibited antibacterial activity by hydrolyzing bacterial cell walls.
  • Enzymatic activity remained stable for at least one week.

Conclusions:

  • APS is a viable and sustainable method for producing biocatalytic membranes.
  • This approach simplifies enzyme immobilization compared to traditional post-production methods.
  • The developed membranes offer a promising platform for applications requiring integrated biological functions.