Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Gastrulation01:56

Gastrulation

59.3K
Gastrulation establishes the three primary tissues of an embryo: the ectoderm, mesoderm, and endoderm. This developmental process relies on a series of intricate cellular movements, which in humans transforms a flat, “bilaminar disc” composed of two cell sheets into a three-tiered structure. In the resulting embryo, the endoderm serves as the bottom layer, and stacked directly above it is the intermediate mesoderm, and then the uppermost ectoderm. Respectively, these tissue strata...
59.3K
Determination01:51

Determination

19.4K
During embryogenesis, cells become progressively committed to different fates through a two-step process: specification followed by determination. Specification is demonstrated by removing a segment of an early embryo, “neutrally” culturing the tissue in vitro—for example, in a petri dish with simple medium—and then observing the derivatives. If the cultured region gives rise to cell types that it would normally generate in the embryo, this means that it is specified. In...
19.4K
Neurulation01:30

Neurulation

43.0K
Neurulation is the embryological process which forms the precursors of the central nervous system and occurs after gastrulation has established the three primary cell layers of the embryo: ectoderm, mesoderm, and endoderm. In humans, the majority of this system is formed via primary neurulation, in which the central portion of the ectoderm—originally appearing as a flat sheet of cells—folds upwards and inwards, sealing off to form a hollow neural tube. As development proceeds, the...
43.0K
Non-Canonical Wnt Signaling Pathways01:41

Non-Canonical Wnt Signaling Pathways

7.6K
Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
7.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

First Measurement of Time-Dependent CP Violation in the Flavor-Changing Neutral-Current Decay B^{0}→K_{S}^{0}μ^{+}μ^{-}.

Physical review letters·2026
Same author

Measurement of the Top-Quark Production Cross Section and Charge Asymmetry at LHCb.

Physical review letters·2026
Same author

Searches for B^{0}→K^{+}π^{-}τ^{+}τ^{-} and B_{s}^{0}→K^{+}K^{-}τ^{+}τ^{-} Decays.

Physical review letters·2026
Same author

[Nomogram incorporating OCT-based plaque morphology and computational physiology features for predicting major adverse cardiovascular events in patients with acute coronary syndrome].

Zhonghua xin xue guan bing za zhi·2026
Same author

First Evidence of the B_{s}^{0}→K^{-}π^{+}γ Decay.

Physical review letters·2026
Same author

Precision Measurement of CP Violation and Branching Fractions in B^{±}→K_{S}^{0}h^{±} (h=π, K) Decays and Search for the Rare Decay B_{c}^{±}→K_{S}^{0}K^{±}.

Physical review letters·2026

Related Experiment Video

Updated: Oct 1, 2025

Analyzing Craniofacial Morphogenesis in Zebrafish Using 4D Confocal Microscopy
09:16

Analyzing Craniofacial Morphogenesis in Zebrafish Using 4D Confocal Microscopy

Published on: January 30, 2014

11.2K

Fn1 Regulates the Third Pharyngeal Pouch Patterning and Morphogenesis.

X Wang1, Y Liang1, Z Zhu2

  • 1Department of Anatomy and Histology, Shenzhen University Health Science Center, Shenzhen, China.

Journal of Dental Research
|March 9, 2022
PubMed
Summary

Fibronectin 1 (Fn1), produced by neural crest cells (NCCs), is crucial for thymus and parathyroid development. Loss of Fn1 in NCCs disrupts third pharyngeal pouch patterning, leading to organ abnormalities.

Keywords:
developmentendodermfibronectinneural crestparathyroid glandsthymus glands

More Related Videos

Isolation of Whole Cell Protein Lysates from Mouse Facial Processes and Cultured Palatal Mesenchyme Cells for Phosphoprotein Analysis
07:26

Isolation of Whole Cell Protein Lysates from Mouse Facial Processes and Cultured Palatal Mesenchyme Cells for Phosphoprotein Analysis

Published on: April 1, 2022

2.0K
Two-step Approach to Explore Early- and Late-stages of Organ Formation in the Avian Model: The Thymus and Parathyroid Glands Organogenesis Paradigm
13:43

Two-step Approach to Explore Early- and Late-stages of Organ Formation in the Avian Model: The Thymus and Parathyroid Glands Organogenesis Paradigm

Published on: June 17, 2018

7.6K

Related Experiment Videos

Last Updated: Oct 1, 2025

Analyzing Craniofacial Morphogenesis in Zebrafish Using 4D Confocal Microscopy
09:16

Analyzing Craniofacial Morphogenesis in Zebrafish Using 4D Confocal Microscopy

Published on: January 30, 2014

11.2K
Isolation of Whole Cell Protein Lysates from Mouse Facial Processes and Cultured Palatal Mesenchyme Cells for Phosphoprotein Analysis
07:26

Isolation of Whole Cell Protein Lysates from Mouse Facial Processes and Cultured Palatal Mesenchyme Cells for Phosphoprotein Analysis

Published on: April 1, 2022

2.0K
Two-step Approach to Explore Early- and Late-stages of Organ Formation in the Avian Model: The Thymus and Parathyroid Glands Organogenesis Paradigm
13:43

Two-step Approach to Explore Early- and Late-stages of Organ Formation in the Avian Model: The Thymus and Parathyroid Glands Organogenesis Paradigm

Published on: June 17, 2018

7.6K

Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Genetics

Background:

  • The parathyroid and thymus glands originate from the third pharyngeal pouch.
  • Neural crest cells (NCCs) are essential mesenchymal components interacting with endodermal cells during pharyngeal pouch development.
  • The precise role of NCCs in regulating third pharyngeal pouch development is not fully understood.

Purpose of the Study:

  • To investigate the function of fibronectin 1 (Fn1) synthesized by NCCs in the development of the third pharyngeal pouch.
  • To elucidate the molecular mechanisms by which NCC-derived Fn1 influences thymus and parathyroid morphogenesis.

Main Methods:

  • Utilized genetic manipulation to study the effects of Fn1 loss in NCCs during embryonic development.
  • Analyzed gene expression patterns (Foxn1, Bmp4, Tbx1, Fgf10) and signaling pathway activity (Hedgehog signaling).
  • Examined organogenesis and morphology of thymus and parathyroid derivatives in mutant embryos.

Main Results:

  • Loss of Fn1 in NCCs resulted in decreased Foxn1 expression in the presumptive thymus.
  • Hedgehog signaling was upregulated in the presumptive parathyroid, while Bmp4 expression decreased in the presumptive thymus.
  • Ectopic expansion of Tbx1 and downregulation of Fgf10 were observed in the mutant.
  • Mutant embryos exhibited abnormal thymus and parathyroid development, including hypoparathyroidism, hypoplastic thymus, and ectopic organs.

Conclusions:

  • Fibronectin 1 (Fn1) synthesized by NCCs is critical for the proper patterning of the third pharyngeal pouch.
  • Fn1 regulates NCC functions essential for thymus and parathyroid morphogenesis.
  • Disruption of Fn1 in NCCs leads to significant developmental defects in thymus and parathyroid derivatives.