MiR-129-2-3p Inhibits Esophageal Carcinoma Cell Proliferation, Migration, and Invasion via Targeting DNMT3B

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Updated: Oct 1, 2025

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice
07:02

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice

Published on: August 23, 2019

7.5K

Xuyang Peng1, Xuhui Wu1, Gongzhi Wu1

  • 1Department of Cardiothoracic Surgery, The Sixth Affiliated Hospital of Wenzhou Medical University, Lishui, 323000, China.

Current Molecular Pharmacology
|March 9, 2022
PubMed
Abstract

Insights

MicroRNA-129-2-3p acts as a tumor suppressor in esophageal carcinoma (EC) by targeting DNMT3B. This finding offers new therapeutic strategies for EC treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Esophageal carcinoma (EC) is a significant global health concern.
  • Understanding the molecular mechanisms driving EC progression is crucial for developing effective treatments.

Purpose of the Study:

  • To investigate the role of microRNA-129-2-3p (miR-129-2-3p) in esophageal carcinoma.
  • To elucidate the regulatory mechanism of miR-129-2-3p in EC cell progression.
  • To identify potential therapeutic targets for EC.

Main Methods:

  • Analysis of miRNA and RNA sequencing data from the TCGA-ESCA dataset.
  • Prediction of miRNA targets using the StarBase database.
  • Experimental validation of miR-129-2-3p and DNMT3B expression, their interaction, and functional impact on EC cells using qRT-PCR, Western blot, CCK-8, scratch healing, Transwell, and dual-luciferase assays.

Main Results:

  • MiR-129-2-3p was found to be significantly downregulated in EC cell lines, while DNMT3B expression was upregulated.
  • MiR-129-2-3p directly targets and binds to DNMT3B.
  • Overexpression of miR-129-2-3p inhibited EC cell progression, an effect reversed by simultaneous overexpression of DNMT3B.

Conclusions:

  • MiR-129-2-3p functions as a tumor suppressor in esophageal carcinoma.
  • The tumor-suppressive activity of miR-129-2-3p in EC is mediated through its targeting of DNMT3B.
  • These findings highlight miR-129-2-3p as a potential therapeutic agent for esophageal carcinoma.
Keywords:
DNMT3Besophageal carcinomainvasionmiR-129-2-3pmigrationproliferation

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