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Updated: Oct 1, 2025

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Published on: September 28, 2022
A novel method for transforming Geobacillus kaustophilus with a chromosomal segment of Bacillus subtilis transferred
Kotaro Mori1, Kaho Fukui1, Ryotaro Amatsu1
1Department of Science, Technology and Innovation, Kobe University, 1-1 Rokkodai, Nada, Kobe, 657 5801, Japan.
Background:
Geobacillus kaustophilus is a thermophilic Gram-positive bacterium. Methods for its transformation are still under development. Earlier studies have demonstrated that pLS20catΔoriT mobilized the resident mobile plasmids from Bacillus subtilis to G. kaustophilus and transferred long segments of chromosome from one cell to another between B. subtilis.
Results:
In this study, we applied mobilization of the B. subtilis chromosome mediated by pLS20catΔoriT to transform G. kaustophilus. We constructed a gene cassette to be integrated into G. kaustophilus and designed it within the B. subtilis chromosome. The pLS20catΔoriT-mediated conjugation successfully transferred the gene cassette from the B. subtilis chromosome into the G. kaustophilus allowing for the desired genetic transformation.
Conclusions:
This transformation approach described here will provide a new tool to facilitate the flexible genetic manipulation of G. kaustophilus.
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