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Related Experiment Video

Updated: Oct 1, 2025

Analysis of Cardiomyocyte Development using Immunofluorescence in Embryonic Mouse Heart
10:56

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Published on: March 26, 2015

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SIRT1 regulates cardiomyocyte alignment during maturation.

Yi Fang1, Wei Fan1, Xiaojiang Xu2

  • 1Signal Transduction Laboratory, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA.

Journal of Cell Science
|March 9, 2022
PubMed
Summary

Sirtuin 1 (SIRT1) is crucial for cardiomyocyte alignment and heart development. SIRT1 deficiency disrupts cardiomyocyte organization and function, impacting cardiac maturation.

Keywords:
AlignmentCardiomyocyte maturationChemotaxisContractionSIRT1

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Area of Science:

  • Cardiovascular Biology
  • Developmental Biology
  • Molecular Cardiology

Background:

  • Cardiomyocyte alignment is essential for proper heart function, enabling organized contraction.
  • The molecular mechanisms controlling cardiomyocyte alignment during development are not fully understood.

Purpose of the Study:

  • To investigate the role of Sirtuin 1 (SIRT1) in regulating cardiomyocyte alignment and cardiac remodeling during development.

Main Methods:

  • Utilized an in vitro differentiation system using human embryonic stem cells.
  • Generated SIRT1-deficient mouse models at late developmental stages.
  • Analyzed cardiomyocyte/myofibril alignment, beating patterns, and gene expression related to chemotaxis pathways (CXCL12/CXCR4, CCL2/CCR2/CCR4).

Main Results:

  • SIRT1 deficiency in vitro impaired cardiomyocyte/myofibril alignment and disrupted beating patterns.
  • SIRT1 deletion in mouse embryos led to irregular cardiomyocyte distribution, myofibril misalignment, and reduced heart size.
  • SIRT1 deficiency blunted the expression of key chemotaxis genes, and CCL2 signaling inhibition reduced cardiomyocyte alignment.

Conclusions:

  • SIRT1 plays a critical role in controlling cardiomyocyte alignment at the inter-cellular level during cardiac maturation.
  • SIRT1 influences cardiac development by regulating gene expression in chemotaxis pathways, impacting cardiomyocyte organization and heart size.