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Related Experiment Video

Updated: Oct 1, 2025

Electrospinning Growth Factor Releasing Microspheres into Fibrous Scaffolds

Electrospinning Growth Factor Releasing Microspheres into Fibrous Scaffolds

Published on: August 16, 2014

Electrosprayed Regeneration-Enhancer-Element Microspheres Power Osteogenesis and Angiogenesis Coupling.

Tianpeng Xu¹, Yuhe Yang¹, Di Suo¹

¹Department of Biomedical Engineering, The Hong Kong Polytechnic University, Hung Hom, Hong Kong SAR, P. R. China.

Small (Weinheim an Der Bergstrasse, Germany)

|March 9, 2022

Summary

New electrosprayed microspheres enhance bone regeneration by releasing L-arginine and nitric oxide (NO). This system stimulates both encapsulated and surrounding cells, promoting bone healing and new blood vessel formation.

Keywords:

angiogenesis bone regeneration electrosprayed microspheres osteogenesis regeneration-enhancer-element

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Last Updated: Oct 1, 2025

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Visualizing Angiogenesis by Multiphoton Microscopy In Vivo in Genetically Modified 3D-PLGA/nHAp Scaffold for Calvarial Critical Bone Defect Repair

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Published on: April 19, 2015

Area of Science:

Biomaterials Science
Regenerative Medicine
Tissue Engineering

Background:

Conventional bone regeneration strategies often lack effective osteogenic modulation for encapsulated and surrounding cells.
Existing methods face challenges in coordinating stem cell activity and vascularization at bone defect sites.

Purpose of the Study:

To develop electrosprayed microspheres as regeneration-enhancer-element reservoirs for improved bone healing.
To investigate the internal/external modulation loop created by encapsulated cells and released factors.

Main Methods:

Electrospraying of sodium alginate microspheres encapsulating L-arginine doped hydroxyapatite nanoparticles (Arg/HA NPs) and bone mesenchymal stem cells (BMSCs).
Utilizing Arg/HA NPs as reservoirs for L-arginine and Ca2+.
Investigating the metabolic conversion of L-arginine to nitric oxide (NO) by BMSCs and its signaling pathway activation (NO/cyclic guanosine monophosphate/cGMP).

Main Results:

Demonstrated effective bone tissue formation and neovasculature increase in rat calvarial defect models.
Showcased the dual role of microspheres in providing internal cell modulation and external enhancement for surrounding cells.
Confirmed the promotion of osteogenesis-angiogenesis coupling through released NO, Ca2+, and L-arginine.

Conclusions:

The developed Arg/HA-SA@BMSC microspheres serve as effective regeneration-enhancer-element reservoirs.
This system establishes an internal/external cell modulation loop, significantly enhancing bone regeneration.
The microsphere system offers a promising strategy for high throughput, minimally invasive, and effective vascularized bone regeneration therapy.