Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

513
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
513
CRISPR01:59

CRISPR

53.2K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
53.2K
CRISPR and crRNAs02:53

CRISPR and crRNAs

17.6K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dual-loaded homotypic exosomes with endogenous IR808 and pH-responsive DOX drive sequenced chemo-PDT and convert "cold" OSCC tumors "hot".

Journal of nanobiotechnology·2026
Same author

A robust RNAi nanoplatform for precise activation of cGAS-STING pathway and effective immune checkpoint blockade to potentiate cancer immunotherapy.

Journal of controlled release : official journal of the Controlled Release Society·2026
Same author

PRL-3 up-regulates exosomal ITGαvβ5 expression to promote liver pre-metastatic niche formation and colon cancer liver metastasis.

Experimental hematology & oncology·2026
Same author

SIRT5-RAC2 Axis Drives Monocyte-to-Macrophage Differentiation to Promote Inflammatory Injury in Premature Ovarian Insufficiency.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Co-Delivery of Chemotherapy and Anti-Angiogenic Lipid via DPPA-LNPs Potentiates Anti-PD-1 Immunotherapy.

International journal of nanomedicine·2025
Same author

Corrigendum to "Repolarization of macrophages to improve sorafenib sensitivity for combination cancer therapy" [Acta Biomaterialia 162 (2023) 98-109].

Acta biomaterialia·2025

Related Experiment Video

Updated: Oct 1, 2025

CRISPR/Cas9 Ribonucleoprotein-mediated Precise Gene Editing by Tube Electroporation
08:31

CRISPR/Cas9 Ribonucleoprotein-mediated Precise Gene Editing by Tube Electroporation

Published on: June 20, 2019

14.2K

Nanoparticles-Mediated CRISPR/Cas Gene Editing Delivery System.

Phei Er Saw1, Guo-Hui Cui2, Xiaoding Xu1

  • 1Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Guangdong-Hong Kong Joint Laboratory for RNA Medicine, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, 107 Yanjiang West Road, Yuexiu District, Guangzhou, Guangdong, China.

Chemmedchem
|March 9, 2022
PubMed
Summary

Efficient delivery of CRISPR/Cas gene editing systems using nanoparticles is crucial for treating genetic diseases. This review explores new nanoparticle strategies for CRISPR/Cas delivery and their clinical applications in gene therapy.

Keywords:
CRISPR/Casgene editinggene therapynanoparticles

More Related Videos

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

34.5K
Delivery of the Cas9/sgRNA Ribonucleoprotein Complex in Immortalized and Primary Cells via Virus-like Particles "Nanoblades"
09:23

Delivery of the Cas9/sgRNA Ribonucleoprotein Complex in Immortalized and Primary Cells via Virus-like Particles "Nanoblades"

Published on: March 31, 2021

5.2K

Related Experiment Videos

Last Updated: Oct 1, 2025

CRISPR/Cas9 Ribonucleoprotein-mediated Precise Gene Editing by Tube Electroporation
08:31

CRISPR/Cas9 Ribonucleoprotein-mediated Precise Gene Editing by Tube Electroporation

Published on: June 20, 2019

14.2K
Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

34.5K
Delivery of the Cas9/sgRNA Ribonucleoprotein Complex in Immortalized and Primary Cells via Virus-like Particles "Nanoblades"
09:23

Delivery of the Cas9/sgRNA Ribonucleoprotein Complex in Immortalized and Primary Cells via Virus-like Particles "Nanoblades"

Published on: March 31, 2021

5.2K

Area of Science:

  • Biomedical research
  • Gene therapy
  • Nanotechnology

Background:

  • CRISPR/Cas technology offers significant potential for treating genetic diseases.
  • Efficient delivery of CRISPR/Cas components to target cells remains a major challenge.
  • Nanoparticles are emerging as promising delivery vehicles for CRISPR/Cas systems.

Purpose of the Study:

  • To review the development of CRISPR/Cas delivery systems.
  • To explore novel strategies for enhancing CRISPR/Cas delivery.
  • To discuss the clinical applications of these delivery systems in gene-related diseases.

Main Methods:

  • Literature review of recent advancements in CRISPR/Cas delivery systems.
  • Analysis of nanoparticle-based strategies for CRISPR/Cas delivery.
  • Examination of current and potential clinical applications.

Main Results:

  • Nanoparticles demonstrate significant potential for efficient and targeted delivery of CRISPR/Cas systems.
  • Various nanoparticle formulations are being developed to overcome delivery barriers.
  • Successful preclinical studies highlight the therapeutic promise of nanoparticle-delivered CRISPR/Cas.

Conclusions:

  • Advancements in nanoparticle technology are critical for realizing the full potential of CRISPR/Cas gene therapy.
  • Novel delivery strategies are paving the way for effective clinical translation of CRISPR/Cas for genetic disorders.
  • Continued research in this area is expected to drive innovation in gene therapy.