Mameliella sediminis sp. nov., a novel polyhydroxyalkanoate-accumulating bacterium

A Gram-stain-negative, strictly aerobic, non-motile, rod-shaped bacterium, capable of producing poly-β-hydroxyalkanoate, designated DP3N28-2^T, was isolated from the sediment collected from Daya Bay, Guangdong, PR China. Optimal growth occurred at 37-40 °C, pH 6.0 and in the presence of 4 % NaCl. The 16S rRNA gene sequences analysis revealed that DP3N28-2^T showed highest similarities with Mameliella alba DSM 23384^T (98.3 %), Antarctobacter jejuensis 13-2-B6^T (97.2 %), Antarctobacter heliothermus El-219^T (96.8 %), Maliponia aquimaris MM-10^T (96.7 %), Ponticoccus litoralis CL-GR66^T (96.4 %) and Aquicoccus porphyridii L1 8-17^T (96.1 %). The predominant fatty acids (>10 %) were summed feature 8 (C_18 : 1ω6c and/or C_18 : 1ω7c; 72.1 %) and C_16 : 0 (11.0 %). The polar lipids contain phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylglycerol, one aminophosphlipid, one phospholipid and three unidentified lipids. The respiratory quinone was Q-10. The DNA G+C content was 63.0 mol% (data from the genome sequence). The estimated genome size was 5.12 Mb. The average nucleotide identity values between the DP3N28-2^T genome and the genome of M. alba was 81.1 %, while the digital DNA-DNA hybridization value was 23.4 %. The phenotypic, genotypic and chemotaxonomic differences between DP3N28-2^T and its phylogenetic relatives indicates that DP3N28-2^T should be regarded as representing a novel species of the genus Mameliella, for which the name Mameliella sediminis sp. nov. is proposed. The type strain is DP3N28-2^T (=MCCC 1K06218^T=KCTC 82804^T).