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Updated: Oct 1, 2025

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E2F4 may be a core transcription factor in the lncRNA-TF regulatory network in cervical cancer

Haiyan Yang1,2, Xiaoli Qu3, Jiangang Huang4

  • 1Department of Obstetrics Gynecology, Zhujiang Hospital, Southern Medical University, Guangzhou, China.

Journal of Clinical Laboratory Analysis
|March 9, 2022
PubMed
Abstract

Insights

This study reveals that the long non-coding RNA-transcription factor (lncRNA-TF) regulatory network is crucial in cervical cancer development. E2F4 is identified as a key transcription factor within this network, offering potential targets for precise therapies.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genomics

Background:

  • Cervical cancer is a leading cause of gynecological cancer mortality globally.
  • Current therapeutic strategies for cervical cancer have limitations, necessitating further research into its regulatory mechanisms.
  • Identifying novel gene targets and targeted therapies is crucial for improving treatment outcomes.

Purpose of the Study:

  • To investigate the regulatory network mechanisms underlying cervical cancer.
  • To identify potential gene targets for precise targeted therapy in cervical cancer.
  • To explore the role of differentially expressed long non-coding RNAs (DELs) and their regulatory networks.

Main Methods:

  • Microarray analysis of three paired cervical squamous cell carcinoma and noncancerous tissues.
  • Screening of differentially expressed mRNAs (DEMs) and DELs (|fold change| ≥ 2, p < 0.05).
  • Functional annotation and pathway analysis of DEMs; functional prediction and cis/trans-regulatory network analysis of DELs.

Main Results:

  • Functional prediction of DELs indicated significant enrichment in cytosol (Cellular Component), mitotic cell cycle (Molecular Function), and protein binding (Biological Process).
  • Cis-regulation analysis predicted 96 DELs involved in cis-regulatory relationships.
  • lncRNA-trans-regulation network analysis identified E2F4 as a potential core transcription factor in cervical cancer.

Conclusions:

  • The lncRNA-TF regulatory network significantly influences the occurrence and progression of cervical cancer.
  • E2F4 emerges as a critical transcription factor within the identified lncRNA-TF regulatory network.
  • These findings suggest E2F4 as a potential therapeutic target for cervical cancer treatment.
Keywords:
LncRNAcervical cancermicroarraytranscription factor

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