Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Diversity of Antigen Receptors01:28

Diversity of Antigen Receptors

858
Antigen receptors are essential components of the immune system crucial in defending the body against foreign invaders. These receptors are present on the surface of B and T cells, enabling them to recognize antigens and mount an appropriate immune response.
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
858
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

7.0K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
7.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Peripheral kappa opioid receptors modulate cold hypersensitivity in a mouse model of chemotherapy-induced neuropathy.

Pain·2026
Same author

N-Branched Tricyclic Guanidines as Novel Melanocortin-3 Receptor Agonists and Melanocortin-4 Receptor Antagonists.

Journal of medicinal chemistry·2025
Same author

Structure-Activity Relationship of Pyrrolidine Pentamine Derivatives as Inhibitors of the Aminoglycoside 6'-<i>N</i>-Acetyltransferase Type Ib.

Antibiotics (Basel, Switzerland)·2024
Same author

Ag-driven CD8 + T cell clonal expansion is a prominent feature of MASH in humans and mice.

Hepatology (Baltimore, Md.)·2024
Same author

Structure-activity relationship of pyrrolidine pentamine derivatives as inhibitors of the aminoglycoside 6'- <i>N</i> -acetyltransferase type Ib.

bioRxiv : the preprint server for biology·2024
Same author

Antigen-driven CD8 <sup>+</sup> T cell clonal expansion is a prominent feature of MASH in humans and mice.

bioRxiv : the preprint server for biology·2024

Related Experiment Video

Updated: Oct 1, 2025

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope
08:09

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope

Published on: March 24, 2017

9.7K

Identification of B Cell and T Cell Epitopes Using Synthetic Peptide Combinatorial Libraries.

Clemencia Pinilla1, Marc A Giulianotti1, Radleigh G Santos2

  • 1Center for Translational Science, Florida International University, Port St. Lucie, Florida.

Current Protocols
|March 9, 2022
PubMed
Summary

This study introduces a combinatorial peptide library method for identifying B and T cell epitopes. The approach efficiently screens peptide mixtures to find targets recognized by antibodies and T cells.

Keywords:
B cellB cell epitopesT cellT cell epitopesantigen discoveryepitopespeptide ligands

More Related Videos

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes
07:59

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes

Published on: March 25, 2014

15.1K
Peptide:MHC Tetramer-based Enrichment of Epitope-specific T cells
13:58

Peptide:MHC Tetramer-based Enrichment of Epitope-specific T cells

Published on: October 22, 2012

18.2K

Related Experiment Videos

Last Updated: Oct 1, 2025

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope
08:09

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope

Published on: March 24, 2017

9.7K
A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes
07:59

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes

Published on: March 25, 2014

15.1K
Peptide:MHC Tetramer-based Enrichment of Epitope-specific T cells
13:58

Peptide:MHC Tetramer-based Enrichment of Epitope-specific T cells

Published on: October 22, 2012

18.2K

Area of Science:

  • Immunology
  • Biochemistry
  • Synthetic Chemistry

Background:

  • Identifying specific B and T cell epitopes is crucial for vaccine development and understanding immune responses.
  • Current methods for epitope discovery can be time-consuming and resource-intensive.
  • Combinatorial library approaches offer a high-throughput alternative for epitope mapping.

Purpose of the Study:

  • To present a novel combinatorial library method for the synthesis and screening of peptide mixtures.
  • To identify B and T cell epitopes recognized by monoclonal antibodies (MAbs) and T cells, respectively.
  • To provide detailed protocols for library preparation, screening, and optimization.

Main Methods:

  • Synthesis and screening of mixture-based synthetic combinatorial peptide libraries (hexapeptides and decapeptides).
  • Positional scanning peptide library approach for identifying antibody-recognized epitopes using competitive ELISA.
  • Screening decapeptide libraries to identify T cell peptide ligands by assessing T cell activation.
  • Optimization of assay conditions for MAbs and T cells to ensure sensitivity and reproducibility.

Main Results:

  • Demonstrated the efficacy of hexapeptide libraries in identifying antigenic determinants recognized by MAbs.
  • Showcased the utility of decapeptide libraries in identifying T cell peptide ligands.
  • Established protocols for library preparation, screening, and deconvolution of epitope information.

Conclusions:

  • The presented combinatorial library method provides an efficient strategy for discovering B and T cell epitopes.
  • This approach facilitates the identification of key peptide sequences for immunological applications.
  • The protocols offer a robust framework for epitope mapping and characterization.