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VIM-encoding IncpSTY plasmids and chromosome-borne integrative and mobilizable elements (IMEs) and integrative and

Fangzhou Chen1,2,3, Peng Wang1, Zhe Yin1

  • 1State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, 100071, China.

Annals of Clinical Microbiology and Antimicrobials
|March 10, 2022
PubMed
Abstract

Insights

Carbapenem-resistance blaVIM genes in Pseudomonas are spread via mobile genetic elements (MGEs). This study details five such MGEs, revealing novel IncpSTY plasmids and diverse resistance genes, enhancing understanding of VIM-encoding MGE evolution.

Area of Science:

  • Microbiology
  • Genetics
  • Molecular Biology

Background:

  • Carbapenem-resistance genes blaVIM are prevalent in Pseudomonas.
  • These genes are often found within class 1 integrons and mobile genetic elements (MGEs).
  • Limited detailed genetic information exists for blaVIM-carrying MGEs in Pseudomonas.

Purpose of the Study:

  • To present the complete genetic sequences of five blaVIM-2/-4-carrying MGEs from Pseudomonas.
  • To analyze the genetic structure and diversity of these MGEs, including plasmids, integrative and mobilizable elements (IMEs), and integrative and conjugative elements (ICEs).
  • To investigate the role of these MGEs in the dissemination of antibiotic resistance genes.

Main Methods:

  • Sequencing of five blaVIM-2/-4-carrying MGEs (two plasmids, two IMEs, one ICE).
  • Comparative sequence analysis of 15 MGEs from four different groups.
  • Identification and characterization of resistance genes and integrons within the MGEs.

Main Results:

  • Two novel plasmids belonging to the IncpSTY incompatibility group were identified.
  • A total of 15 MGEs were analyzed, including previously unidentified elements.
  • At least 22 resistance genes (antibiotics and heavy metals) were found, with most in accessory modules.
  • Eleven MGEs carried blaVIM genes within distinct class 1 integrons.

Conclusions:

  • blaVIM-carrying integrons were integrated into mobile plasmids, IMEs, and ICEs.
  • These MGEs facilitate the intercellular transfer and spread of blaVIM genes among Pseudomonas isolates.
  • The findings provide insights into the genetic diversification and evolution of VIM-encoding MGEs, aiding bacterial survival under antibiotic stress.
Keywords:
Antibiotic resistanceBla VIMIntegronsMobile genetic elementsPseudomonasST129

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