Study on the Relationship between lncRNA Gene Polymorphism and Systemic Lupus Erythematosus
Xue Guan1, Dan Liu1, Yuling Xing2
1Clinical Laboratory, First Affiliated Hospital of Harbin Medical University, 150001, China.
Computational and Mathematical Methods in Medicine
|March 10, 2022
Summary
This study found altered expression of specific long-chain noncoding RNAs (lncRNAs) in systemic lupus erythematosus (SLE) patients. Lnc0640 was overexpressed, while lnc3643 showed decreased levels and correlated with disease activity and symptoms.
Area of Science:
- Immunology
- Molecular Biology
- Genetics
Background:
- Systemic lupus erythematosus (SLE) is a complex autoimmune disease affecting multiple organs.
- Long-chain noncoding RNAs (lncRNAs) are implicated in the pathogenesis of various autoimmune conditions.
Purpose of the Study:
- To quantify the expression levels of five specific lncRNAs in SLE patients compared to healthy controls.
- To investigate the correlation between lncRNA expression and clinical manifestations and laboratory markers in SLE.
Main Methods:
- A case-control study design involving 76 SLE patients and 71 healthy controls.
- Quantitative real-time polymerase chain reaction (qPCR) was employed to measure lncRNA expression in peripheral blood mononuclear cells (PBMCs).
- Statistical analysis using SPSS 23.0 with a significance level of α = 0.05.
Main Results:
- lnc0640 expression was significantly higher in SLE patients than in controls (P=0.03).
- lnc5150 expression was significantly lower in SLE patients (P<0.001).
- lnc3643 expression was lower in SLE patients, particularly those with rash or pleurisy (P<0.05), and negatively correlated with SLE disease activity score (SLEDAI-2K), ESR, and CRP (P<0.05).
Conclusions:
- lnc0640 is overexpressed in SLE PBMCs.
- lnc3643 expression is decreased in SLE and associated with disease activity and specific clinical symptoms like arthritis, rash, and pleuritis.
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