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Single-Cell Transcriptome Comparison of Bladder Cancer Reveals Its Ecosystem
Yongxiang Luo1,2, Tao Tao1,2, Ran Tao1
1Institute of Urological Surgery, The Third Affiliated Hospital of Shenzhen University, Shenzhen University, Shenzhen, China.
Frontiers in Oncology
|March 10, 2022
Summary
Single-cell sequencing reveals distinct tumor microenvironments in bladder cancer (BLCA). Different cell populations and activated pathways offer potential biomarkers for predicting clinical outcomes in BLCA patients.
Area of Science:
- Oncology
- Genomics
- Immunology
Background:
- Bladder carcinoma (BLCA) is a heterogeneous cancer with poorly understood biological behaviors.
- Understanding the tumor microenvironment (TME) is crucial for BLCA progression and treatment.
Purpose of the Study:
- To profile the cellular and molecular landscape of BLCA using single-cell RNA sequencing.
- To identify differences in TME composition and pathway activation across various BLCA grades and cystitis glandularis.
Main Methods:
- Single-cell RNA sequencing was performed on four clinical BLCA samples.
- Analysis of 26,792 cell transcriptomes to characterize cellular composition and activated biological pathways.
Main Results:
- N-glycan biosynthesis pathway was activated in high-grade BLCA, while TNF-related pathways were active in cystitis glandularis.
- TME composition varied significantly: T cells dominated cystitis glandularis, macrophages were prevalent in low- and high-grade tumors, and stromal cells were abundant in high-grade relapse.
- Significant heterogeneity was observed in the TME of different BLCA samples.
Conclusions:
- Single-cell transcriptome profiles provide insights into cystitis glandularis and BLCA in different clinical states.
- Identified biological programs and TME compositions may serve as potential biomarkers for clinical prognosis in BLCA.
