Related Experiment Video

Updated: Sep 30, 2025

Quantitative Immunohistochemistry of the Cellular Microenvironment in Patient Glioblastoma Resections
05:45

Quantitative Immunohistochemistry of the Cellular Microenvironment in Patient Glioblastoma Resections

Published on: July 31, 2017

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The Role of m6A Regulator-Mediated Methylation Modification and Tumor Microenvironment Infiltration in Glioblastoma

Liang Wang1, Haiyan Cao1, Ying Zhong2

  • 1Department of Neurosurgery, Tangdu Hospital of Fourth Military Medical University, Xi'an, China.

Frontiers in Cell and Developmental Biology
|March 10, 2022
PubMed

Insights

N6-methyladenosine (m6A) RNA methylation impacts the Glioblastoma tumor microenvironment (TME). Low m6A scores correlate with better patient outcomes and therapeutic advantages, suggesting m6A patterns influence TME cell infiltration and stemness.

Area of Science:

  • Epigenetics
  • Cancer Biology
  • Immunology

Background:

  • N6-methyladenosine (m6A) RNA methylation is a key epigenetic mechanism.
  • Epigenetic regulation of immune responses is established, but its role in Glioblastoma (GBM) tumor microenvironment (TME) remains unclear.
  • The impact of m6A on GBM TME cell infiltration and stemness is largely unknown.

Purpose of the Study:

  • To investigate the role of m6A modification patterns in GBM TME cell infiltration and stemness.
  • To develop a quantitative score (m6Ascore) for m6A modification patterns in individual GBM samples.
  • To correlate m6A modification patterns with TME characteristics and stemness.

Main Methods:

  • Comprehensive evaluation of m6A modification patterns in 310 GBM samples using 21 m6A regulators.
  • Systematic correlation analysis between m6A patterns, TME cell infiltration, and stemness characteristics.
  • Construction of m6Ascore using principal component analysis.

Main Results:

  • Identified two distinct m6A modification patterns associated with GBM immunophenotypes (immune activation vs. immune desert).
  • Discovered two modes of m6A methylation regulating immunity and stemness, with high m6Ascore linked to stromal activation and poor immune infiltration.
  • Pan-cancer analysis confirmed m6AScore's correlation with clinical outcome, immune infiltration, and stemness.

Conclusions:

  • m6A modifications significantly contribute to TME complexity and stemness diversity in GBM.
  • Patients with low m6Ascore exhibit improved therapeutic advantages and clinical benefits.
  • Assessing m6A patterns can enhance understanding of TME infiltration and stemness, aiding immunotherapeutic strategy development.
Keywords:
GBMTMEm6Amethylationnovel immunotherapypan-cancer

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