Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

High-Performance Liquid Chromatography: Types of Detectors01:15

High-Performance Liquid Chromatography: Types of Detectors

863
The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte...
863
Qualitative Analysis01:10

Qualitative Analysis

729
Qualitative analysis is the process of identifying elements, ions, or compounds in an unknown sample. It is the first and most fundamental type of analysis based on the hierarchy of analytical goals. This hierarchy is significant as it provides a structured approach to scientific research, with qualitative analysis serving as the initial step, providing essential information before moving on to quantitative or other forms of analysis.
There are two main approaches to qualitative analysis:...
729
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

922
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
922
Volatilization01:10

Volatilization

597
Volatilization gravimetry is an analytical technique that measures the mass lost due to the volatilization of the substance. This technique is used to estimate the amount of volatile material in a sample. To perform this method, heat a known amount of the sample to a high temperature in a crucible or other suitable vessel. The volatile substance in the sample evaporates, and the vapor is completely expelled from the crucible either by heating the sample or bubbling a stream of inert gas through...
597
Sample Preparation for Analysis: Overview01:21

Sample Preparation for Analysis: Overview

386
Sample preparation is an essential step in the analytical process. It involves preparing a sample so that it can be analyzed accurately. The goal is to extract the analyte, the substance you want to measure, from the sample while removing any components that may interfere with the analysis. Sample preparation techniques vary depending on the physical state of the sample.
Bulk or large solid samples are typically reduced in size using grinding, crushing, or milling techniques to increase the...
386
Gas Chromatography: Types of Detectors-II01:19

Gas Chromatography: Types of Detectors-II

544
In gas chromatography, different detectors are employed to meet specific analytical needs. These detectors are often categorized based on their detection mechanisms and the types of compounds they are best suited to analyze. Thermal Conductivity Detectors (TCD), Flame Ionization Detectors (FID), and Electron Capture Detectors (ECD) represent common categories, each with unique operating principles and applications. However, beyond these, several other detectors are designed for more specialized...
544

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Microwave-assisted sample preparation: A review of 50 years of achievements and trends.

Analytica chimica acta·2026
Same author

One-pot iron determination in fortified flours using thymol-1,10-phenanthroline deep eutectic solvent and smartphone-based digital image photometry.

Analytical methods : advancing methods and applications·2026
Same author

Revisiting Catalytic Methods Exploiting Digital Videos: Sequential Determination of Copper and Sucrose in Sugarcane Spirits.

ACS omega·2025
Same author

Determination of volatile acids in sugarcane spirits exploring an air drag system and digital image photometry.

Analytical methods : advancing methods and applications·2025
Same author

An improved approach for analysis of chocolate by laser induced breakdown spectroscopy (LIBS) using L-menthol as matrix modifier.

Talanta·2025
Same author

Natural deep eutectic solvent as extractor and support for determination of metals by laser induced breakdown spectroscopy.

Analytica chimica acta·2025
Same journal

RETRACTED: Atta et al. Effect of Montmorillonite Nanogel Composite Fillers on the Protection Performance of Epoxy Coatings on Steel Pipelines. <i>Molecules</i> 2017, <i>22</i>, 905.

Molecules (Basel, Switzerland)·2026
Same journal

Correction: Chen et al. Chemical Composition of <i>Litsea pungens</i> Essential Oil and Its Potential Antioxidant and Antimicrobial Activities. <i>Molecules</i> 2023, <i>28</i>, 6835.

Molecules (Basel, Switzerland)·2026
Same journal

Correction: Ruan et al. Comparison of Extraction, Isolation, Purification, Structural Characterization and Immunomodulatory Activity of Polysaccharides from Two Species of <i>Cistanche</i>. <i>Molecules</i> 2025, <i>30</i>, 4754.

Molecules (Basel, Switzerland)·2026
Same journal

Correction: Li et al. Gastrodin Ameliorates Cognitive Dysfunction in Vascular Dementia Rats by Suppressing Ferroptosis via the Regulation of the Nrf2/Keap1-GPx4 Signaling Pathway. <i>Molecules</i> 2022, <i>27</i>, 6311.

Molecules (Basel, Switzerland)·2026
Same journal

Correction: Zueva et al. Steady-State Kinetics of Enzyme-Catalyzed Hydrolysis of Echothiophate, a P-S Bonded Organophosphorus as Monitored by Spectrofluorimetry. <i>Molecules</i> 2020, <i>25</i>, 1371.

Molecules (Basel, Switzerland)·2026
Same journal

1,4-Diazatriphenylene and Its Hetero-Fused Analogs: Synthesis and Applications.

Molecules (Basel, Switzerland)·2026
See all related articles

Related Experiment Video

Updated: Sep 30, 2025

Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns
06:25

Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns

Published on: April 26, 2016

15.3K

Chemical Derivatization in Flow Analysis.

Fábio R P Rocha1, Elias A G Zagatto1

  • 1Center for Nuclear Energy in Agriculture, University of Sao Paulo, Piracicaba 13416-000, Brazil.

Molecules (Basel, Switzerland)
|March 10, 2022
PubMed
Summary
This summary is machine-generated.

Chemical derivatization enhances analytical chemistry, especially in flow analysis, by improving detection sensitivity and selectivity. Proper flow system design is crucial to avoid signal distortions and baseline noise for accurate measurements.

Keywords:
Schlieren effectUV-Vis spectrophotometryflow analyzersflow programminggreen analytical chemistrykinetic discriminationmanifold programmingsolid-phase reagents

More Related Videos

Workflow Based on the Combination of Isotopic Tracer Experiments to Investigate Microbial Metabolism of Multiple Nutrient Sources
12:47

Workflow Based on the Combination of Isotopic Tracer Experiments to Investigate Microbial Metabolism of Multiple Nutrient Sources

Published on: January 22, 2018

9.5K
Metabolic Pathway Confirmation and Discovery Through 13C-labeling of Proteinogenic Amino Acids
07:26

Metabolic Pathway Confirmation and Discovery Through 13C-labeling of Proteinogenic Amino Acids

Published on: January 26, 2012

24.5K

Related Experiment Videos

Last Updated: Sep 30, 2025

Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns
06:25

Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns

Published on: April 26, 2016

15.3K
Workflow Based on the Combination of Isotopic Tracer Experiments to Investigate Microbial Metabolism of Multiple Nutrient Sources
12:47

Workflow Based on the Combination of Isotopic Tracer Experiments to Investigate Microbial Metabolism of Multiple Nutrient Sources

Published on: January 22, 2018

9.5K
Metabolic Pathway Confirmation and Discovery Through 13C-labeling of Proteinogenic Amino Acids
07:26

Metabolic Pathway Confirmation and Discovery Through 13C-labeling of Proteinogenic Amino Acids

Published on: January 26, 2012

24.5K

Area of Science:

  • Analytical Chemistry
  • Spectrophotometry
  • Luminometry

Background:

  • Chemical derivatization is vital for enhancing selectivity and sensitivity in analytical chemistry.
  • Flow analysis offers reproducible reagent addition and controlled timing, making it ideal for derivatization.
  • Advanced techniques leverage kinetic discrimination and Green Analytical Chemistry principles.

Purpose of the Study:

  • To review flow-based chemical derivatization strategies in analytical chemistry.
  • To discuss methods for improving selectivity and sensitivity using flow analysis.
  • To highlight techniques for circumventing signal anomalies in flow systems.

Main Methods:

  • Exploitation of flow and manifold programming for derivatization.
  • Utilization of solid-phase reagents and novel sample/reagent addition strategies.
  • Focus on spectrophotometric and luminometric detection methods.

Main Results:

  • Flow-based derivatization enables kinetic discrimination and use of unstable reagents.
  • Strategies exist to increase sample residence time and improve reproducibility.
  • Proper flow system design can prevent Schlieren effects and baseline noise.

Conclusions:

  • Flow-based chemical derivatization is a powerful tool for sensitive and selective analysis.
  • Careful system design is essential to mitigate artifacts and ensure reliable data.
  • This review critically assesses current approaches in spectrophotometric and luminometric flow analysis.