Optimization of Biocompatibility for a Hydrophilic Biological Molecule Encapsulation System

Area of Science:

• Biotechnology
• Materials Science
• Pharmaceutical Sciences

Background:

• Challenges in biologic drug delivery and storage hinder clinical applications.
• Protein destabilization is a major issue in current encapsulation methods.
• Decylmonoacyl glycerol (10MAG) and lauryldimethylamino-N-oxide (LDAO) reverse micelles in alkanes preserve biomacromolecule stability.

Purpose of the Study:

• To develop a biocompatible solvent system for 10MAG/LDAO reverse micelles for biologic drug storage and delivery.
• To screen and identify optimal biocompatible solvents for the 10MAG/LDAO system.
• To lay the foundation for room-temperature storage and novel delivery systems for biologics.

Main Methods:

• A novel screening approach was used to evaluate the 10MAG/LDAO system.
• Seven biocompatible solvents were tested across two preparation methods.
• Toxicity and encapsulation efficiency were analyzed using a hydrophilic small molecule, followed by preliminary protein encapsulation tests.

Main Results:

• Optimal solvent properties for the 10MAG/LDAO system were identified.
• The study demonstrated the feasibility of using biocompatible solvents for reverse micelle formulation.
• Predictions from small molecule screening were validated with initial protein encapsulation tests.

Conclusions:

• The developed 10MAG/LDAO system with biocompatible solvents shows promise for biologic drug storage and delivery.
• This research provides a foundation for future advancements in long-term, room-temperature biologic storage.
• The findings pave the way for innovative water-in-oil-in-water biologic delivery systems.